用快速高效液相色谱系统分离纯化Gc蛋白

报告Gc蛋白的一种分离方法，为免疫制备抗Gc血清制备抗原。硫酸该分级沉淀出含Gc的人血清组份，BlueSepharoseCL－6B亲和色谱除去含Gc硫酸铸分级沉淀的人血清组份中的白蛋白，快速高效液相色谱系统过Alkyl-SuperoseTMHR5／5疏水色谱柱和MonoQHR5／5离子交换柱。聚丙烯酸胺凝胶解离、非解离电泳证明，Gc蛋白得到了彻底纯化。免疫固定显示：纯化出的蛋白确实是Gc蛋白．为1-1型。快速高效液相色谱为蛋白质的纯化提供了新的技术及仪器手段。

ISOLATION AND PURIFICATION OF Gc PROTEIN BY FAST PERFORMANCE LIQUID CHROMATOGRAPHY SYSTEM

Group Gc protein fractions were obtained by addition of ammonium sulfate (saturation including 35%~ 75%) to human plasma and follwed by The affinity chromatography on a selfmade column packed with Blue Sepharose CL-6B,hydrophobic interaction chromatography on an AlkylxSuperoseTM HR5/5 column and ion exchange chromatography on a MonoQ HR5/5 column by the FPLC system.After these processes,SDS and non-reduced polyacrylamide gel slab electrophoreses were carried out.The results showed that the Gc protein was purified. Furtherrnore, the double agar diffusion test demonstrated that the purified protein was Gc protein belonging to type 1-1.