| 2008～2009年浙江省猪圆环病毒2型全基因组序列的比较和分析 |
| |
| 引用本文： | 张茜茜,朱炳林,方维焕.2008～2009年浙江省猪圆环病毒2型全基因组序列的比较和分析[J].中国兽医科学,2010,40(6). |
| |
| 作者姓名： | 张茜茜 朱炳林 方维焕 |
| |
| 作者单位： | 浙江大学,动物预防研究所浙江省动物预防医学重点实验室,浙江,杭州,310029 |
| |
| 摘 要： | 应用PCR方法对2008～2009年采集的99份疑似断奶仔猪多系统衰竭综合征病猪的脾、肺、淋巴结混合组织样本进行猪圆环病毒2型(PCV2)检测,并以组织DNA为模板进行了PCV2的全基因组扩增,克隆后进行了测序。结果显示,2008年与2009年浙江省PCV2的平均阳性率为47.5%,与2004～2006年相比基本持平。随机选取18份阳性病料进行PCV2全基因组测序分析,结果,18个毒株均属于Group1,其中5个毒株(27.8%)属于1A分支,3个毒株(16.7%)属于1B分支,9个毒株(50%)属于1C分支。表明,Group1依然是浙江省PCV2的主导基因型,且1C分支从无到有,并逐渐占据主导地位。
|
| 关 键 词： | 猪圆环病毒2型 全基因组测序 分子流行病学 |
Analysis and comparison of genomic sequences of porcine circovirus type 2 isolates in Zhejiang Province between 2008 and 2009 |
| |
| ZHANG Qian-qian,ZHU Bing-lin,FANG Wei-huan.Analysis and comparison of genomic sequences of porcine circovirus type 2 isolates in Zhejiang Province between 2008 and 2009[J].Veterinary Science in China,2010,40(6). |
| |
| Authors: | ZHANG Qian-qian ZHU Bing-lin FANG Wei-huan |
| |
| Institution: | ZHANG Qian-qian,ZHU Bing-lin,FANG Wei-huan(Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine/Institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,China) |
| |
| Abstract: | Porcine circovirus type2(PCV2) specific PCR method was used to detect 99 mixed tissue samples,including spleen,lung and lymph nodes of diseased pigs from 2008 to 2009.Out of the 99 samples,47 were PCV2 positive(47.5%),which was at similar level to that from 2004 to 2006.Eighteen PCV2 isolates were then randomly selected for sequencing and analysis.Among 18 PCV2 isolates of Group 1,5 belonged to subgroup 1A(27.8%),3 to subgroup 1B(16.7%),and 9 to subgroup 1C(50%).Group 1 remained to be the predominant PCV2 g... |
| |
| Keywords: | porcine circovirus type 2 genome sequencing molecular epidemiology |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
