| 8个miniSTR基因座复合扩增系统的建立及其应用 |
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| 引用本文： | 赵贺群,姜维斌,于蛟,刘锋,姜先华.8个miniSTR基因座复合扩增系统的建立及其应用[J].中国法医学杂志,2009,24(2):83-86. |
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| 作者姓名： | 赵贺群 姜维斌 于蛟 刘锋 姜先华 |
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| 作者单位： | 辽宁省刑事科学技术研究所,辽宁,沈阳,110032 |
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| 摘 要： | 目的建立扩增片段〈200bp，包含CSF1PO，TH01，TPOX，D3S1358，FGA，D7S820，D21S11，PentaD 8个miniSTR基因座的复合扩增体系。方法采用四色荧光染料标记引物，PCR扩增后，应用ABI 3130遗传分析仪进行片段长度分析，对280例无关个体，137例疑难生物物证进行了检验。结果280例无关个体的调查结果为除1例在CSF1PO基因座外，其余样本的各基因座分型结果与AmpFLSTR Identifiler试剂盒完全相同。与应用ID试剂盒比较，明显提高了137例疑难生物物证的检出率。结论该检测技术方法稳定，结果准确，重复性好，且其判型结果可进行DNA数据库查询、比对，为刑事案件及灾难事故中疑难生物物证的检验提供了一条新的途径。
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| 关 键 词： | 法医物证学 miniSTR 荧光标记 复合扩增 个体识别 |
The establishment of a 8-locus miniSTR fluorescent-multiplex PCR and its application in the analysis of degraded DNA |
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| ZHAO Hequn,JIANG Weibin,YU Jiao,LIU Feng,JIANG Xianhua.The establishment of a 8-locus miniSTR fluorescent-multiplex PCR and its application in the analysis of degraded DNA[J].Chinese Journal of Forensic Medicine,2009,24(2):83-86. |
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| Authors: | ZHAO Hequn JIANG Weibin YU Jiao LIU Feng JIANG Xianhua |
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| Institution: | ZHAO Hequn,JIANG Weibin,YU Jiao,LIU Feng,JIANG Xianhua/ Liaoning Forensic Science Institute,Shenyang 110032,China |
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| Abstract: | Objective To establish a 8-locus miniSTR fluorescent-multiplex PCR for forensic purposes, in which all of the amplified fragments are shorter than 200bp. Methods Eight highly polymorphic STR loci, CSF1PO, TH01, TPOX, D3S1358, FGA, D7S820, D21S11 and Penta D, were selected, and a 8-locus miniSTR fluorescent-multiplex PCR system was developed through redesigning primers, labeling fluorescence dye, and optimizing experiment conditions. Then, 280 unrelated individuals from Liaoning Han population and 137 highly... |
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| Keywords: | miniSTR |
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