Profiler Plus试剂盒扩增Amelogenin基因座变异1例

目的探讨日常检案及DNA数据库建设中常用的ProfilerPlus系统扩增法医生物检材可能出现的变异情况。方法分别采用ProfilerPlus试剂盒扩增、毛细管电泳分析及Amelogenin单基因座扩增、银染技术分型两种方法分别检测两份血痕的基因型。结果应用ProfilerPlus试剂盒扩增、毛细管电泳分析发现X-Y同源Amelogenin基因座的X片段丢失,而运用Amelogenin单基因座扩增、银染技术分型则X片段正常出现。结论应用ProfilerPlus系统分析法医生物检材,有可能出现等位基因丢失现象,此现象需要引起我们在日常检案及DNA数据库建设中的足够重视。

Analysis of a variation case when using the Profiler Plus kit to amplify the Amelogenin loci

Objective To investigate the possibility of variation when usually using the Profiler Plus kit to amplify the forensic biological samples in DNA database construction and case work.Methods Two blood stains from a criminal case are typed by two methods: 1) using Profiler Plus kit to amplify the samples and capillary electrophoresis technique to analyze the PCR products;2)using single amelogenin gene amplifying technique to amplify the samples and the silver dying technique to analyze the PCR products.Results Profiler Plus typing results show X-Specific products dropout at the amelogenin loci in the two samples, and single amelogenin gene amplifying and typing results show the normal X- and Y- Specific products.Conclusion When applying the Profiler Plus system to analyze the forensic biological samples ,the phenomenon that alleles dropout probably appears, which asks us to pay attention to it very much in DNA database construction and case work .