Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra‐High‐Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection |
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| Authors: | Mei Wang PhD Yan‐Hong Wang PhD Bharathi Avula PhD Mohamed M Radwan PhD Amira S Wanas PhD Zlatko Mehmedic MSc John van Antwerp BSc Mahmoud A ElSohly PhD Ikhlas A Khan PhD |
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| Institution: | 1. National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, USA;2. Department of Pharmacognosy, Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt;3. Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt;4. Waters Corporation, Milford, MA, USA;5. Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, Oxford, MS, USA;6. Division of Pharmacognosy, Department of BioMolecular Science, School of Pharmacy, University of Mississippi, Oxford, MS, USA;7. Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia |
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| Abstract: | Ultra‐high‐performance supercritical fluid chromatography (UHPSFC ) is an efficient analytical technique and has not been fully employed for the analysis of cannabis. Here, a novel method was developed for the analysis of 30 cannabis plant extracts and preparations using UHPSFC /PDA ‐MS . Nine of the most abundant cannabinoids, viz . CBD , ?8‐THC , THCV , ?9‐THC , CBN , CBG , THCA ‐A, CBDA , and CBGA , were quantitatively determined (RSD s < 6.9%). Unlike GC methods, no derivatization or decarboxylation was required prior to UHPSFC analysis. The UHPSFC chromatographic separation of cannabinoids displayed an inverse elution order compared to UHPLC . Combining with PDA ‐MS , this orthogonality is valuable for discrimination of cannabinoids in complex matrices. The developed method was validated, and the quantification results were compared with a standard UHPLC method. The RSD s of these two methods were within ±13.0%. Finally, chemometric analysis including principal component analysis (PCA ) and partial least squares‐discriminant analysis (PLS ‐DA ) were used to differentiate between cannabis samples. |
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| Keywords: | forensic science Cannabis sativa L cannabinoids UHPSFC/PDA‐MS quantification and method validation chemometric analysis |
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