| 荧光复合扩增检测3个Y—STR基因座单倍型 |
| |
| 作者姓名： | Lin XY Wang L Yuan BL Feng CJ Huang DX |
| |
| 作者单位： | 1. 华中科技大学同济医学院法医学系,湖北,武汉,430030
2. 郑州市公安局刑侦支队,河南,郑州,450003 |
| |
| 摘 要： | 目的建立检测3个Y-STR基因座Y-GATA-A7.1、DYS456和DYS443的荧光复合扩增体系,并获取中国汉族人群单倍型频率分布。方法用荧光标记引物对郑州地区203名汉族男性无关个体进行3个基因座复合扩增,ABI3100型遗传分析仪检测、分型。结果Y-GATA-A7.1、DYS456和DYS443基因座分别检出5、6和6个等位基因,其基因多样性(GD值)分别为0.6692、0.5839和0.7053。三个基因座构成的单倍型共有44种,单倍型多样性(HD值)为0.9523。结论建立的3个Y-STR基因座荧光标记复合扩增系统具有很高的识别能力,可应用于法医学实践。
|
| 关 键 词： | Y-STRs 复合扩增 单倍型 Y-GATA-A7.1 DYS456 DYS443 |
| 文章编号： | 1004-5619(2006)02-0122-03 |
| 修稿时间： | 2005年6月21日 |
Detecting the haplotype of three Y-STR loci by fluorescent multiplex amplification |
| |
| Lin XY,Wang L,Yuan BL,Feng CJ,Huang DX.Detecting the haplotype of three Y-STR loci by fluorescent multiplex amplification[J].Journal of Forensic Medicine,2006,22(2):122-124. |
| |
| Authors: | Lin Xiao-Yan Wang Lei Yuan Bao-Long Feng Chang-Jun Huang Dai-Xin |
| |
| Institution: | Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. linziily@yahoo.com.cn |
| |
| Abstract: | Objective To establish a fluorescent multiplex PCR system for typing Y-STR loci Y-GATA-A7.1, DYS456 and DYS443, and investigate their haplotype frequencies in Chinese Han population. Methods 203 unrelated males of Han population living in Zhengzhou were typed by fluorescent multiplex amplification system and ABI 3100 genetic analyzer. Results In Zhengzhou Han population, 5,6 and 6 different alleles were observed for Y-GATA-A7.1, DYS456 and DYS443 loci, and their gene diversity (GD) were 0.669 2, 0.583 9 and 0.705 3 respectively. A total of 44 different haplotypes formed by these three loci was identified and the haplotype diversity (HD) reached 0.952 3. Conclusion The fluorescent multiplex system for these three Y-STR loci will be very powerful for forensic individual identification and paternity testing in Chinese Han population. |
| |
| Keywords: | Y-STRs multiplex amplification haplotype Y-GATA-A7 1 DYS456 DYS443 |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
|
