| 人体组织黄递酶Ⅲ分型 |
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| 引用本文： | 黄秋菊,杨庆恩.人体组织黄递酶Ⅲ分型[J].中国法医学杂志,1994(3). |
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| 作者姓名： | 黄秋菊 杨庆恩 |
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| 作者单位： | 武汉同济医科大学法医学系!武汉，430030，武汉同济医科大学法医学系!武汉，430030 |
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| 摘 要： | 应用超薄层PAGIEF对人体组织作DIA3分型，结果显示睾丸、卵巢、子宫肌和牙髓组织均可检出DIA3多型带。脑、肝、肾、肾上腺、脾、肠、胰腺、心肌、肺、皮肤和骨骼肌以及血液、阴道液和毛根均未检测出DIA3。应用紫外分光光度法测定人体不同组织，血液和阴道液中DIA相对活性，结果各组织DIA活性相对值与PAGIEF中DIA显色谱带密度基本一致。作者认为，用PAGIEF对生殖器官和牙髓进行DIA3分型在法医的个人识别中有一定意义，在混合斑鉴定中可以不考虑阴道液对精液DIA3分型的干扰。
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| 关 键 词： | 黄递酶Ⅲ（DIA_3） 人体组织 等电聚焦（IEF） |
DIA_3 TYPING OF HUMAN TISSUES |
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| Huang Qiuju , Yang Qingen.DIA_3 TYPING OF HUMAN TISSUES[J].Chinese Journal of Forensic Medicine,1994(3). |
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| Authors: | Huang Qiuju Yang Qingen |
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| Abstract: | DIA3 typing for human tissues was carried out by IEF- The results showed that the DIA3 typescould be demonstrated in testis, ovary, uterus and dental pulps ;but could not be demonstrated in thehuman brain, liver, kidney, adrenal gland, spleen, intestine, pancrease, heart, lung, skin, muscle, blood,vaginal secretion and hair root. The relative activty of DIA in different human tissues,blood and vagi-nal secretion DIA activity of different tissues and the DIA band intensity by PAGIEF were basecallyconsistent. The author were examined by spectrophotometric analysis. The relative suggests that theDIA3 typing of reproductive organs and teeth by PAGIEF plays a significant role for medicolegal individualization,The vaginal sectetion did not interfere with DIA3 typing of semen in mixed stain for theindividualization. |
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| Keywords: | Diaphorase Ⅲ (DIA_3) Human Tissue IEF |
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