| 大鼠急性心肌缺血再灌流损伤c-fos mRNA原位杂交实验研究 |
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| 作者姓名： | Ma MY Xu X |
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| 作者单位： | 1. 深圳市公安局刑科所,广东,深圳,518008
2. 汕头大学医学院,广东,汕头,515031 |
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| 基金项目： | 国家自然科学基金(No.39700167) |
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| 摘 要： | 目的 探讨急性心肌缺血和/或再灌流早期不同时间不同区域心肌细胞内原癌基因c-fos mRNA表达变化,为心肌早期缺血死后诊断提供依据。方法 复制心肌早期缺血模型,大鼠分为正常、缺血及缺血再灌流组。采用原位杂交方法结合图像分析与统计学处理方法研究心肌细胞内c-fos mRNA表达情况。结果 缺血10min再灌流30min,部分大鼠再灌流区心肌细胞胞浆内c-fos mRNA弱阳性反应,散在分布。缺血60min再灌流30min时达高峰,120min开始减弱。正常和单纯缺血组中缺血少于60min组未见有阳性表达。HE染色无明显病理改变。结论 原位杂交法检测c-fos mRNA方法灵敏特异,有望成为一项急性心肌缺血/再灌流早期损伤的诊断指标。
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| 关 键 词： | 心肌缺血 再灌流 原位杂交 c-fos mRNA |
| 文章编号： | 1004-5619(2003)02-0065-03 |
| 修稿时间： | 2003年1月7日 |
Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats |
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| Ma MY,Xu X.Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats[J].Journal of Forensic Medicine,2003,19(2):65-67,F003. |
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| Authors: | Ma Meng-yun Xu Xiaohu |
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| Institution: | Shenzhen Criminal Science & Technology Institute, Shenzhen 518008, China. |
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| Abstract: | Objective To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/ R) during acute period. Methods The model of I/R was established,and the rats was divided normal control,is-chmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes. Results After 10min ischemia and 30min reperfusion,the area of reperfusion showed a part of cardiac myocytes weak staining,and get to peak at I-60min/R-30min. The myocardium in normal control and ischemia groups less than 60min showed negative staining. No changes were found in all groups by HE staining. Conclusion c-fos mRNA detection may become an important method for diagnosis of I/R. |
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| Keywords: | myocardial ischemia/reperfusion(I/R) c-fos mRNA in situ hybridization |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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