Comparison of DNA typing using AmpFlSTR Yfiler and PowerPlex Y System,for specimens subject to very long storage |
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| Affiliation: | 1. Immunological Research, Universy of Cartagena, Cartagena, Colombia;2. Laboratorio GENES Ltda, Medellín, Colombia;3. Instituto de Biología, Universidad de Antioquia, Medellín, Colombia;4. Laboratorio de Genética Molecular, Cruz Roja Ecuatoriana, Quito, Ecuador;5. Universidad Pontificia Bolivariana, Medellín, Colombia;6. IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Portugal;1. Laboratorio de Genética Molecular de Cruz Vital – Cruz Roja Ecuatoriana Quito, Ecuador;2. Laboratorio GENES Ltda, Medellin, Colombia;1. Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA Laboratory, Budapest, Hungary;2. Research Centre for Natural Sciences of the HAS, Department of Complex Systems, Budapest, Hungary |
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| Abstract: | We performed DNA typing from blood stains stored at room temperature for 22–30 years, using AmpFlSTR Yfiler PCR amplification Kit and PowerPlex Y System to extract and amplify the DNA and performing electrophoresis with an ABI 310 Genetic Analyzer. We identified alleles using GeneMapper ID v3.2.1 software. We found that long-term storage tended to reduce the number of detectable loci in the blood stains and that it was easier to detect the loci of smaller amplicons than larger amplicons. |
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| Keywords: | Y-STR AmpFlSTR Yfiler PowerPlex Y Degradation Blood Stain |
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