Developmental validation of 15 X chromosomal short tandem repeat markers |
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| Institution: | 1. Armed Forces DNA Identification Laboratory, Armed Forces Medical Examiner System, United States;2. American Registry of Pathology, United States;3. Flinders University, Australia;4. National Institute of Standards and Technology, United States;1. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany;2. IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal;3. Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain;1. Laboratorio de Genética Molecular de Cruz Vital – Cruz Roja Ecuatoriana Quito, Ecuador;2. Laboratorio GENES Ltda, Medellin, Colombia;1. Department of Anthropology, 1621 Cumberland Avenue, 502A Strong Hall, University of Tennessee, Knoxville, TN, 37996-1525, United States;2. International Commission on Missing Persons Headquarters, Koninginnegracht 12 2514 AA Den Haag, The Netherlands;3. Tyrolpath, Klostergasse 1, 6511 Zams, Austria;4. Defense POW/MIA Accounting Agency, 590 Moffet St., Bldg 4077, Joint Base Pearl Harbor-Hickam, HI 96853, United States;1. School of Forensic and Investigative Science, University of Central Lancashire, Preston, UK;2. Key Forensic Services, DNA Division, Norwich, UK |
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| Abstract: | The use of X chromosomal short tandem repeat (STR) markers has been greatly increasing in the forensic setting. Using guidelines set forth previously for the validation of autosomal and Y STRs, aspects of the feasibility of routine X chromosomal STR use were evaluated. Two mini-X chromosomal STR multiplexes capable of amplifying 15 total markers were developed and utilized to determine allele nomenclature, allele/genotype frequencies, mutation rates, and linkage between markers. Additionally, a concordance study between these multiplexes and a commercially available kit was performed. Here, the authors present an overview of this extensive developmental validation study. |
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| Keywords: | X chromosome Short tandem repeat Multiplex development Mutation rate Linkage Population database |
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