Touch DNA collection from improvised explosive devices: A comprehensive study of swabs and moistening agents |
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| Institution: | 1. DNA Analysis Center, Scientific Crime Detection Division 10, Royal Thai Police, Thailand;2. Forensic Science Program, Department of Applied Science, Faculty of Science, Prince of Songkla University, Thailand;3. Institute of Forensic Medicine, Royal Thai Police, Thailand;4. Central Scientific Crime Detection Division, Royal Thai Police, Thailand;1. Forensic Genetic Service, Centre Branch, National Institute of Legal Medicine and Forensic Sciences, P.I., Coimbra, Portugal;2. CENCIFOR, Forensic Sciences Centre, Portugal;3. Department of Biology, University of Aveiro, Portugal;4. Faculty of Biomedical Sciences “Abel Salazar”, Porto, Portugal;5. Centre Branch of National Institute of Legal Medicine and Forensic Sciences, P.I., Coimbra, Portugal;6. Medicine Faculty of the University of Coimbra, Portugal;1. Dipartimento di Scienze Biomediche e Sanità Pubblica, Sezione di Medicina Legale, Università Politecnica delle Marche, Ancona, Italy;2. Dipartimento di Sanità Pubblica, Neuroscienze, Medicina Sperimentale e Forense, Sezione di Medicina Legale e Scienze Forensi, Università di Pavia, Italy;3. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Germany;4. Dipartimento di Scienze della Salute, Sezione di Medicina Legale, Università di Genova, Italy;1. Victoria Police Forensic Services Centre, Macleod, Victoria 3085, Australia;2. LaTrobe University, Bundoora, Victoria 3086, Australia;1. Fingerprint Database Laboratory, Division of Identification and Forensic Science, Israel Police, Jerusalem, Israel;2. Biology & DNA Laboratory, Division of Identification and Forensic Science, Israel Police, Jerusalem, Israel;3. Biological Anthropology & Ancient DNA Laboratory, Hadassah School of Dental Medicine, The Hebrew University, Jerusalem, Israel;4. Laboratory of Environmental Physiology, Hadassah School of Dental Medicine, The Hebrew University, Jerusalem, Israel;5. Casali Center for Applied Chemistry, The Institute of Chemistry, The Hebrew University, Jerusalem, Israel;1. College of Science and Engineering, Flinders University, SA 5042, Australia;2. Forensic Science SA, 21 Divett Place, Adelaide, SA 5000, Australia |
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| Abstract: | Improvised explosive devices (IEDs) are used in devastating terrorist attacks worldwide and daily in Thailand. Touch DNA deposited during IED assembly are subjected to intense heat and pressure, resulting in rare events of usable DNA profiles obtained from real casework. No study has simultaneously evaluated both swab brands and moistening agents for touch DNA collection from substrates encountered in IED evidence. In this study, we investigated the effects of swab brands and moistening agents on DNA collection from adhesive tape, a common IED substrate. A full factorial design using four cotton swab brands (two forensic and two medical cotton swabs) and six moistening agents (DNA-free water, phosphate-buffered saline, ethanol, sodium dodecyl sulfate, isopropanol, and lysis buffer) was employed (24 total combinations). Using buffy coats, we found that DNA recovery depended on both swab brands and moistening agents (p < 0.05). The optimal method recovered significantly higher DNA amount from real IED cases compared to the standard Royal Thai Police method. Percentages of high partial profiles also increased. Our results changed the standard operating protocol of the Thai police. Other commonly found substrates from IED cases are being investigated to maximize the evidential value obtained from touch DNA. |
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| Keywords: | Touch DNA Swab Moistening agent Bombing case |
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