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Profiling pythons to combat common illegal wildlife activities
Affiliation:1. Flinders University, Adelaide, South Australia, Australia;2. Forensic Science SA, Adelaide, South Australia, Australia;1. Laboratorio de Genética Molecular de Cruz Vital – Cruz Roja Ecuatoriana Quito, Ecuador;2. Laboratorio GENES Ltda, Medellin, Colombia;1. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany;2. IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal;3. Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain;1. Armed Forces DNA Identification Laboratory, Armed Forces Medical Examiner System, United States;2. American Registry of Pathology, United States;3. Flinders University, Australia;4. National Institute of Standards and Technology, United States;1. Flinders Centre for Nanoscale Science and Technology, Flinders University, Sturt Road, Bedford Park, Adelaide, South Australia, Australia;2. School of Biological Sciences, Flinders University, Sturt Road, Bedford Park, Adelaide, South Australia, Australia
Abstract:Reptiles are the most common illegally traded vertebrates leading to many species becoming extinct or highly endangered; yet these species receive relatively low publicity and few genetic tests are available. Sought after as unusual pets and trophies, reptilian skin and meat are also valuable commodities, while certain anatomical features are valued in traditional medicinal cultures. The Australian carpet python is a popular target for illegal trade, breeding and export activities. It provides a model example for which to develop forensic markers that will positively impact wildlife criminal investigation and enforcement both in Australia and worldwide. We report on the initial isolation and characterisation of 24 polymorphic Short Tandem Repeat (STR) loci to enable individualisation and paternity testing of carpet pythons. These novel loci have been examined for their polymorphic content, heterozygosity and species specificity. The loci are being arranged into multiplex reactions exhibiting heterozygote balance with subsequent determination of stutter measurements. Allelic frequency databases of native populations are being developed that will allow not only match probabilities but also will potentially predict geographic origin of poached individuals. This profiling system will be subject to rigorous validation for use in legal proceedings.
Keywords:Australian pythons  DNA profiling  Forensic  Individualisation  Multiplex  STR  Transnational crime  Wildlife trade
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