小鼠皮肤切创愈合过程中caspase-3的表达及其活性

目的探讨皮肤切创愈合过程caspase-3的表达、激活情况及其活性的变化规律。方法建立小鼠皮肤切创模型后,应用比色法、Western blotting技术对小鼠皮肤切创愈合过程中不同时间段caspase-3的表达、激活情况及其活性进行研究。结果伤后不同时间的caspase-3活性倍增值、caspase-3酶原表达呈现时间规律性变化,并可见caspase-3的激活片段。结论caspase-3可能在皮肤损伤愈合过程中细胞的凋亡中发挥作用,caspase-3可以成为用于皮肤损伤时间推断的指标。

The Expression of caspase-3 and its Cleavage and Enzymatic Activities during Incised Skin Wound Healing in Mice

Objective To study the expression of procaspase-3 and its activation as well as its enzymatic activity during healing of incised skin wound in mice. Methods The expression of procaspase-3 and its activation were studied on skin incised wound at different posttraumatic intervals in 33 tested mice and 3 control mice by colorimetric measurement of proteolytic activity and Western blotting. Results Caspase-3 activity and procaspse-3 protein levels showed continued increase with prolonged posttraumatic intervals. cleaved P17 fragment of caspase-3 was also detected by Western blotting. Conclusion Our data suggest that caspase-3 appears to play a role in cell apoptosis during the healing process of skin wound, and caspase-3 may be used as a marker for estimation of post-traumatic interval in skin wound.