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Development and evaluation of multiplex Y-STR assays for application in molecular genealogy |
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| Authors: | Manon Jacobs Lynn Janssen Nancy Vanderheyden Bram Bekaert Wim Van de Voorde Ronny Decorte |
| Affiliation: | aUZ Leuven, Department of Forensic Medicine, Laboratory of Forensic Genetics and Molecular Archaeology, Kapucijnenvoer 33, B-3000 Leuven, Belgium;bK.U. Leuven, Department of Human Genetics, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium |
| Abstract: | Three multiplex PCRs were developed for the analysis of 14 single-copy and 4 multi-copy Y chromosome Short Tandem Repeat (STR) loci routinely used by several public genealogical databases. These assays were used in addition to PowerPlex® Y for the analysis of 245 DNA samples from a genealogical project. In total 244 different haplotypes composed of 37–40 alleles were identified with one haplotype identical between two males with the same surname. The multi-copy loci DYS464 and DYS724 were the most polymorphic with a gene diversity of at least 0.964. The use of DYS454 and DYS455 can be questioned as these loci had the lowest gene diversity (0.039 and 0.269, respectively). |
| Keywords: | Multiplex PCR Population genetics Y chromosome Short Tandem Repeat Molecular genealogy |
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