| HLA-A位点DNA分型及其法医学应用 |
| |
| 引用本文： | 刘利民,贾静涛,梁健,U.Gyllensten.HLA-A位点DNA分型及其法医学应用[J].中国法医学杂志,1998(4). |
| |
| 作者姓名： | 刘利民 贾静涛 梁健 U.Gyllensten |
| |
| 作者单位： | 中国医科大学法医血清学教研室!沈阳，110001，中国医科大学法医血清学教研室!沈阳，110001，中国医科大学第一临床学院器官移植科!沈阳，110001，瑞典乌普萨拉大学医学遗传学系 |
| |
| 摘 要： | 应用聚合酶链反应0寡核苷酸探针（PCR－SSO）斑点杂交技术，对222名辽宁地区汉族人群无关个体进行HLA－A基因检测，研究中国辽宁地区汉族群体的HLA－A座位基因分布状况。共检出HLA－A等位基因24个，其中以等位基因HLA－A0201最为常见，频率为0．2635；依次是2402101和1101，等位基因频率分别为0．1847和0．1262.理论杂合度为87％，个人鉴别机率为92％，非父排除率为73．3％。在中国辽宁汉族中检出73种基因型，对观察值和期望值进行X2检验，符合Hardy－Weinberg平衡定律（x2＝6．28，df＝9，0．5＜P＜0．75）。家系分析结果表明按照孟德尔方式遗传。提出的中国辽宁汉族HLA－A等位基因的遗传基因情况，可用于法医学个人识别和亲子鉴定。人类学，HLA相关疾病，及器官移植研究。
|
| 关 键 词： | HLA－A基因分型 多态性 聚合酶链反应（PCR） 寡核苷酸探针 |
DNA typing of HLA-A locus and forensic application |
| |
| Abstract: | The distribution of gene at HLA-A locus was studied in 222 Chinese Han POpulation, using polrmerase chain rea c t ion/ oligon ucleotide probe hybridizatiion technique.24 alleles were found. The most frequent alleIe was the * 0201 (0. 2635). The * 2402101 (0. 1847) and * 1101 (0. 1262) were the next. Consequently the heterozagosity was 87%, while the power of discrimination was 92%. The exclusion probability of paternity was 73.3%. 73 genotypes were detected. The observed genotype distribution conforms withthe expected under the Hardy-Weinberg equilibrium. The data base is useful for the study of HLA-disease association, paternity testing and individual identification as well as anthropology. |
| |
| Keywords: | HLA-A genotyping Polymorphism Polymerase chain reaction (PCR) Oligonucleotide probe |
| 本文献已被 CNKI 等数据库收录！ |
