| 黄连生物碱对HEK293-OATP1B3细胞转运黄芩黄酮活性的影响 |
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| 作者姓名： | 梁 燕 崔馨戈 訾苗苗 汪 杰 刘 艳 王 茜 彭 灿 |
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| 作者单位： | 安徽中医药大学,安徽中医药大学,安徽中医药大学,安徽中医药大学,安徽中医药大学,安徽中医药大学,安徽中医药大学 |
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| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 摘 要： | 目的：探究黄连生物碱对OATP1B3介导的黄芩苷、汉黄芩苷的转运的影响。方法：采用稳定转染OATP1B3的HEK293细胞，以黄芩苷和汉黄芩苷为底物，小檗碱、药根碱、黄连碱、巴马汀作为抑制剂，进行药物转运实验，并进行含量测定，考察黄连生物碱对HEK293-OATP1B3细胞转运黄芩苷和汉黄芩苷的影响。结果：抑制剂组的黄芩苷、汉黄芩苷摄取量均低于对照组（OE组）。结论：黄连生物碱可抑制OATP1B3对黄芩苷和汉黄芩苷的转运。
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| 关 键 词： | 黄连生物碱 黄芩苷 汉黄芩苷 OATP1B3 转运 |
| 收稿时间： | 2022/5/13 0:00:00 |
| 修稿时间： | 2022/10/8 0:00:00 |
Influence of Coptis chinensis Alkaloid on the Activity of HEK293-OATP1B3 Cells in Transport of Scutellaria baicalensis Flavones |
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| Authors: | liangyan cuixinge zimiaomiao wangjie liuyan pengcan and wangqian |
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| Institution: | 1.School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China; 2.Key Laboratory of Chinese Medicinal Formula of Anhui Province,Anhui Hefei 230012,China; 3.Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application,Anhui Hefei 230012,China; 4.Institute of Traditional Chinese Medicine Resources Protection and Development,Anhui Academy of Chinese Medicine,Anhui Hefei 230012,China |
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| Abstract: | Objective: To explore the effect of Rhizoma Coptidis alkaloids on OATP1B3-mediated transport of baicalin and wogonoside. Methods: HEK293 cells stably transfected with OATP1B3 were used to carry out cell uptake experiments with baicalin and wogonoside as substrates and berberine, jatrorrhizine, coptisine, and palmatine as inhibitors. To investigate the effect of Coptis chinensis on the transport of baicalin and wogonoside in HEK293-OATP1B3 cells. Results: The intakes of baicalin and wogonoside in the inhibitor group were lower than those in the control group (OE group). Conclusion: Rhizoma Coptidis alkaloids can inhibit the transport |
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| Keywords: | Rhizoma Coptidis alkaloids baicalin wogonoside OATP1B3 transport |
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