| 针刺对2型糖尿病大鼠脂肪组织INSR基因表达的影响 |
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| 作者姓名： | 蔡辉 袁爱红 魏群利 刘志诚 |
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| 作者单位： | 中国人民解放军南京军区南京总医院中西医结合科;南京中医药大学第二临床医学院; |
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| 基金项目： | 高等学校博士学科点专项科研基金(20040315008) |
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| 摘 要： | 目的探讨针刺对2型糖尿病(type 2 diabetes mellitus,T2DM)机体脂肪组织胰岛素受体(insulinreceptor,INSR)基因表达的影响。方法给食源性肥胖大鼠腹腔注射小剂量链脲佐菌素复制T2DM模型,随机分为针刺组、优降糖组和模型组,设正常对照组。处理4周后,采用罗氏活力型血糖仪测空腹血糖(fast-ing blood sugar,FBS),采用放射免疫法检测空腹胰岛素(fastinginsulin,FINS),采用原位杂交检测脂肪细胞INSRmRNA表达,计算胰岛素敏感性指数(insulin sensitivity index,ISI)和稳态模型评估胰岛素抵抗(homeostasis model assessment of insulin resistance,Homa-IR)指数。结果与正常组比较,模型组FBS、FINS、ISI、Homa-IR水平显著上升(P<0.01),INSR基因水平表达显著降低(P<0.01);与模型组比较,治疗后优降糖组和针刺组FBS、FINS、ISI、Homa-IR显著下降(P<0.05,或P<0.01),INSR基因表达显著升高(P<0.01)。...
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| 关 键 词： | 2型糖尿病 脂肪细胞 胰岛素受体 基因表达 优降糖 针刺 |
Effect of Acupuncture on Insulin Receptor Gene Expression of Adipose Tissue in Type 2 Diabetes Mellitus |
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| Authors: | CAI Hui YUAN Ai-hong WEI Qun-li LIU Zhi-cheng |
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| Institution: | 1.Department of Integrative Traditional Chinese and Western Medicine;the General Hospital of Nanjing Military Area of PLA;Jiangsu Nanjing 210002;China;2.the Second Clinical Medicine College;Nanjing University of Traditional Chinese Medicine;Jiangsu Nanjing 210029;China |
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| Abstract: | Objective To observe effects of acupuncture on the expression of insulin receptor gene(INSR) mRNA of adipocytes in rats with type 2 diabetes mellitus(T2DM).Methods The rat model of T2DM was induced by intraperitoneal injection of small dosage streptozotocin in rats with diet-induced obesity.Then these T2DM rats were averagely randomized into acupuncture group,Glibenclamide group and model group,and normal rats were used as control.After treatment for 4 weeks,fasting blood sugar(FBS) was assayed with active ... |
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| Keywords: | Type 2 diabetes mellitus Adipocytes Insulin receptor Gene expression Glibenclamide Acupuncture |
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