左归饮对肾阴虚衰老大鼠氧化应激反应的影响

目的探讨左归饮水提液延缓肾阴虚大鼠衰老的机制。方法将SD大鼠随机分为5组,分别为正常组,模型组,左归饮高、中、低剂量组,模型组每日灌胃给予甲状腺素混悬液150mg/kg,左归饮高、中、低剂量组分别灌胃左归饮水提液31、20.67、13.78g/kg,连续7d。检测大鼠血清超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、肌酐(creatinine,Cr)、血尿素氮(blood urea nitrogen,BUN)、环磷酸腺苷(cyclic adenosine monophosphate,cAMP)、环磷酸鸟苷(cyclic guanosine monophosphate,cGMP)水平及cAMP/cGMP比值。结果与模型组比较,左归饮高、中、低剂量组大鼠血清cAMP水平,cAMP/cGMP均显著降低(P<0.05),而cGMP水平无显著变化;与模型组比较,左归饮中、高剂量组大鼠血清SOD、CAT、GSH-Px水平均显著升高(P<0.05),MDA水平显著下降(P<0.05);与模型组比较,左归饮低、中、高剂量组大鼠血清Cr和BUN水平均显著降低(P<0.05)。结论左归饮通过抗氧化作用延缓肾阴虚大鼠衰老。

Effect of Zuogui Decoction on Oxidative Stress Response in Senescent Rats with Kidney Yin Deficiency

Objective To investigate the mechanism of water extract of Zuogui Decoction in delaying senescence of rats with kidney Yin deficiency.Methods Sprague-Dawley rats were randomly divided into normal group,model group,and high-,middle-,and low-dose Zuogui Decoction groups.The rats in the model group were given thyroxine suspension 150 mg/kg every day by gavage,and those in the high-,middle-,and low-dose Zuogui Decoction groups were given water extract of Zuogui Decoction at a dose of31,20.67,and 13.78 g/(kg·d),respectively,by gavage.The course of treatment was 7 consecutive days.The levels of superoxide dismutase(SOD),malondialdehyde(MDA),catalase(CAT),glutathione peroxidase(GSH-Px),creatinine(Cr),blood urea nitrogen(BUN),cyclic adenosine monophosphate(cAMP),and cyclic guanosine monophosphate(cGMP)were measured,and cAMP/cGMP ratio was calculated.Results Compared with the model group,the high-,middle-,and low-dose Zuogui Decoction groups had a significant reduction in the serum level of cAMP(P<0.05);the three Zuogui Decoction groups had no significant change in the level of cGMP,with a significant reduction in cAMP/cGMP ratio(P<0.05).Compared with the model group,the middle-and high-dose Zuogui Decoction groups had significant increases in the serum levels of SOD,CAT,and GSH-Px(P<0.05)and a significant reduction in MDA level(P<0.05).Compared with the model group,the low-,middle-,and high-dose Zuogui Decoction groups had significant reductions in the serum levels of Cr and BUN(P<0.05).Conclusion Zuogui Decoction can delay senescence of rats with kidney Yin deficiency by exerting an antioxidant effect.