消化复宁汤对肝郁脾虚型慢性萎缩性胃炎大鼠ERK1/2和p-ERK1/2的影响

目的 研究消化复宁汤对肝郁脾虚型慢性萎缩性胃炎（chronic atrophic gastritis，CAG）大鼠细胞外调节蛋白激酶（extracellular regulated protein kinases，ERK）信号通路的影响，探讨消化复宁汤防治CAG的机制。方法 将80只雄性Wistar大鼠随机取10只为正常组，其余大鼠随机分为模型组，消化复宁汤高、中、低剂量组和维酶素组。采用脱氧胆酸钠及30%和60%无水乙醇灌胃联合施压束缚和饥饱失常法复制肝郁脾虚证CAG模型。实验结束后，观察各组大鼠一般情况的变化，采用ELISA法检测大鼠血清ERK1/2水平，采用Western Blot法检测大鼠胃组织ERK1/2、p-ERK1/2的表达水平。结果 消化复宁汤能明显改善CAG大鼠肝郁脾虚证候表现。消化复宁汤中剂量组与维酶素组血清ERK1/2水平较模型组显著升高（P<0.05），消化复宁汤中、低剂量组与维酶素组大鼠胃组织ERK1/2水平显著高于模型组（P＜0.05）；消化复宁汤高、中、低剂量组及维酶素组大鼠胃组织p-ERK1/2表达水平较模型组显著降低（P＜0.05）；消化复宁汤高、中、低剂量组血清ERK1/2水平和胃组织p-ERK1/2表达水平相比较，差异均无统计学意义（P＞0.05）。结论 消化复宁汤可通过激活ERK1/2，抑制p-ERK1/2的水平防治CAG发生，其作用无明显的剂量依赖性。

Effect of Xiaohua Funing Decoction on ERK1/2 and p-ERK1/2 in Rats with Liver Stagnation and Spleen Deficiency-Type Chronic Atrophic Gastritis

Objective To study the effect of Xiaohua Funing Decoction on the extracellular regulated protein kinase (ERK) signaling pathway in rats with liver stagnation and spleen deficiency (LSSD)-type chronic atrophic gastritis (CAG) and to explore the action mechanism of Xiaohua Funing Decoction in the prevention and treatment of CAG. Methods Ten of 80 male Wistar rats were randomly selected as normal group, and other rats were randomly divided into model group, high-, medium-, and low-dose Xiaohua Funing Decoction groups, and vitacoenzyme group. The model of LSSD-type CAG was established by gavage with sodium deoxycholate and anhydrous ethanol (30% and 60%) combined with pressure/bounding and irregular diet. After the experiment was finished, the changes in general conditions of rats were observed in each group. Serum ERK1/2 level was measured by ELISA, and the expression of ERK1/2 and p-ERK1/2 in gastric tissues was measured by Western blot. Results Xiaohua Funing Decoction significantly improved the LSSD syndrome in rats with CAG. The medium-dose Xiaohua Funing Decoction group and vitacoenzyme group had significantly increased serum ERK1/2 levels compared with the model group (P<0.05), and the medium- and low-dose Xiaohua Funing Decoction groups and vitacoenzyme group had significantly higher ERK1/2 levels in gastric tissues than the model group (P<0.05). The high-, medium-, and low-dose Xiaohua Funing Decoction groups and vitacoenzyme group had significantly reduced expression of p-ERK1/2 in gastric tissues compared with the model group (P<0.05). There were no significant differences in serum ERK1/2 level and gastric p-ERK1/2 expression between the high-, medium-, and low-dose Xiaohua Funing Decoction groups (P>0.05). Conclusion Xiaohua Funing Decoction can prevent and treat CAG by activating ERK1/2 and inhibiting p-ERK1/2 expression, and its effect is not dose-dependent.