| 3种提取胶带粘面汗潜指印中DNA的方法比较 |
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| 引用本文： | 王德明,顾丽华,阎建军,平原,陈荣华,唐建平,郑会芬,糜忠良.3种提取胶带粘面汗潜指印中DNA的方法比较[J].中国法医学杂志,2005,20(2):65-67. |
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| 作者姓名： | 王德明 顾丽华 阎建军 平原 陈荣华 唐建平 郑会芬 糜忠良 |
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| 作者单位： | 上海市公安局刑事科学技术研究所,上海,200083 |
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| 基金项目： | 上海市科技基金资助(03-JG05018) |
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| 摘 要： | 目的比较胶带粘面汗潜指印中DNA提取的方法。方法分别采用硅珠法、QIAMicrokit法、硅珠-QIAMicrokit法提取胶带粘面的汗潜指印中DNA,STR复合扩增,荧光电泳检测。结果用QIAMicrokit法、硅珠-QIAMicrokit法提取胶带粘面汗潜指印中DNA,检测成功率分别为21%和36%。硅珠法检测未获成功。结论硅珠-QIAMicrokit法提取胶带粘面汗潜指印中的DNA比QIAMicrokit法,检验时间更短,检测成功率更高。
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| 关 键 词： | 法医物证学 胶带 汗潜指印 DNA分型 STR分型 |
| 文章编号： | 1001-5728(2005)02-0065-03 |
| 修稿时间： | 2004年6月1日 |
Compare with the three methods of DNA extraction of sweat latent fingerprint on the adhesive side of tapes |
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| WANG De-ming,GU Li-hua,YAN Jian-jun,et al..Compare with the three methods of DNA extraction of sweat latent fingerprint on the adhesive side of tapes[J].Chinese Journal of Forensic Medicine,2005,20(2):65-67. |
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| Authors: | WANG De-ming GU Li-hua YAN Jian-jun |
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| Institution: | WANG De-ming,GU Li-hua,YAN Jian-jun,et al./Shanghai Institute of Forensic Science,Shanghai 200083,China) |
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| Abstract: | Objective To comparatively analyze the DNA extracted from sweat latent fingerprints on the adhesive side of tapes by three kinds of methods. Methods DNA was extracted from sweat latent fingerprint on the adhesive side of tapes using silicon bead test,QIA micro Kit and combined silicon bead-QIA micro Kit. STR loci were detected by multiplex PCR procedures. The PCR product was electrophoresed on an ABI 3100 Genetic Analyzer. Results 36%of the sweat latent fingerprints on the adhesive side of tapes was successfully genotyped using combined silicon bead-QIA micro Kit, and 21% using QIA micro Kit. Conclusion DNA genotyping of the sweat latent fingerprints on the adhesive side of tapes reveals higher possibility using combined silicon bead-QIA micro Kit than using QIA micro Kit and is less time-consuming. |
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| Keywords: | Forensic physical evidence Adhesive tape Sweat latent fingerprint DNA genotype STR genotype |
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