| 实验性小白鼠死后肝脏酶组织化学改变 |
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| 引用本文： | 黄飞骏,刘世沧.实验性小白鼠死后肝脏酶组织化学改变[J].中国法医学杂志,1990(4). |
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| 作者姓名： | 黄飞骏 刘世沧 |
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| 作者单位： | 华西医科大学法医学系 成都
(黄飞骏)，华西医科大学法医学系 成都(刘世沧) |
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| 摘 要： | 作者用60只小鼠实验,分组观察环境温度为4℃和20℃、死后0、6、12、24、48和72h 肝脏的PPr、ATPase、CCO、ACP、ANAE、G-6-Pase、LDH、SDH、G6PD 和NADHD 等10种酶活性的组织化学改变。在4℃组,PPr 活性在死后6h 开始下降;死后72h,PPr 仅在少数肝细胞呈阳性反应;其余9种酶活性无明显改变。在20℃组,死后6h,PPr 活性明显下降,死后12h 变为阴性。死后48h,LDH 和ATPase 活性明显下降,死后72h 变为阴性。作者认为,死后组织中酶的改变规律有助于推断死后经过时间。
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| 关 键 词： | 酶组织化学 死亡时间推断 肝细胞 |
THE POSTMORTEM ENZYME ACTIVITY CHANGES IN MICE'S HEPATIC CELLS |
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| Huang feijun Liu Shicang.THE POSTMORTEM ENZYME ACTIVITY CHANGES IN MICE''''S HEPATIC CELLS[J].Chinese Journal of Forensic Medicine,1990(4). |
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| Authors: | Huang feijun Liu Shicang |
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| Institution: | Huang feijun Liu Shicang Department of pathology faculty of forensic Medicine West China University of Medicine,Changdu |
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| Abstract: | Timing of death is one of the important problems in forensic pathologic practice.Using histochemical methods,the authors observed the enzyme activity changes ofPPr,CCo,ACP,ANAE,LDH,SDH,G6PD,NADHD,G-6-Pase and ATPase inmice liver cells at 0,6,12,24,48,72 hours postmortem(HPM) at the environmen-tal temperature 4℃ and 20℃.The results showed that at 20℃,PPr activity dec-reased markedly at 6 HPM,LDH and ATPase activity decreased at 48 HPM anddisappeared at 72 HPM.The another seven enzymes still showed good enzyme acti-vity at 72 HPM.The authors supposed that the results are significant for timingof postmortem duration. |
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| Keywords: | Enzyme histochemisty Timing of death Liver cell |
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