| 高效液相色谱法测定肝组织中4种蟾蜍二烯内酯类化合物 |
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| 引用本文： | 王朝虹,侯小平,何毅,张继宗,张春水.高效液相色谱法测定肝组织中4种蟾蜍二烯内酯类化合物[J].中国法医学杂志,2003,18(6):323-325. |
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| 作者姓名： | 王朝虹 侯小平 何毅 张继宗 张春水 |
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| 作者单位： | 公安部物证鉴定中心,北京 100038 |
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| 摘 要： | 目的建立高效液相色谱法检测肝组织中蟾蜍二烯内酯的定量方法。方法 以OasisHLB固相萃取柱对样品进行提取,应用HPLC色谱法分离、二极管阵列检测器测定。结果该方法的回收率高于70％,线形范围在12ng/g-1200ng/g,经该方法测得华蟾毒它灵、蟾毒灵的最小检出量为O.4ng,华蟾毒精、脂蟾毒配基的最小检出量为0.5ng(S/N≥3)。结论该方法快速、灵敏、准确,可用于蟾酥中毒的法医学检验。
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| 关 键 词： | 法医毒物学 蟾蜍二烯内酯 高效液相 固相萃取 |
| 文章编号： | 1001-5728(2003)06-0323-03 |
| 修稿时间： | 2003年5月1日 |
Determination of four bufadienolides in human liver by HPLC |
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| WANG Zhaohong,HOU Xiaoping,HE Yi,et al..Determination of four bufadienolides in human liver by HPLC[J].Chinese Journal of Forensic Medicine,2003,18(6):323-325. |
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| Authors: | WANG Zhaohong HOU Xiaoping HE Yi |
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| Institution: | WANG Zhaohong,HOU Xiaoping,HE Yi,et al. /Forensic Science Institute of Public Security Ministry,Beijing 100038 |
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| Abstract: | Objective To establish a method used for quantitative determination of bufadienolides in human liver tissues by HPLC. Methods using solid phase extraction of human liver with Oasis (HLB car-tridge coupled with reserved phase HPLC and diode array detection. Results Recoveries obtained from spiked liver for the bufadienolides were better than 70% . The linearity was studied up to 1200ng/g and the detection limits of the method was 0.4ng for cinobufotalin and bufalin, 0.5ng for cinobufagin and resibufo-genin. Conclusion This method is fast and accurate. It is useful for forensic medicine detection. |
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| Keywords: | Forensic toxicologicai bufadienolides HPLC SPE |
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