| 单细胞凝胶电泳检测大鼠死后肝细胞核DNA降解 |
| |
| 引用本文： | 何远,闫平,胡家伟,孟晓萍.单细胞凝胶电泳检测大鼠死后肝细胞核DNA降解[J].中国法医学杂志,2005,20(6):325-328. |
| |
| 作者姓名： | 何远 闫平 胡家伟 孟晓萍 |
| |
| 作者单位： | 1. 武汉大学医学院法医学教研室,湖北,武汉,430071
2. 武汉市公安局刑侦处,湖北,武汉,430019 |
| |
| 摘 要： | 目的应用单细胞凝胶电泳技术(SCGE)检测大鼠死后肝细胞核DNA降解规律,分析与死亡时间的关系,为早期死亡时间的推断提供新的方法。方法在大鼠死后30h内,每隔3h取肝组织样本进行单细胞凝胶电泳,用共聚焦显微镜摄取彗星图像,应用彗星图像分析软件(IM I1.0)进行图像分析,并作统计学分析。结果死后大鼠的肝细胞在电泳图像上出现明显的彗星形拖尾,其尾长(TL)、尾矩(TM)在一定的时间范围内(0~18h)随死亡时间的延长而逐渐增大,二者均与死亡时间(PM I)呈现一定的相关回归关系。结论单细胞凝胶电泳技术可应用于早期死亡时间的推断。
|
| 关 键 词： | 法医病理学 死亡时间 DNA降解 单细胞凝胶电泳 彗星图像分析软件(IMI1.0) |
| 文章编号： | 1001-5728(2005)06-0325-04 |
| 修稿时间： | 2004年10月25 |
A study on the relationship between nuclear DNA degradation of the rat's liver cells and the postmortem interval by using single cell gel electrophoresis |
| |
| Institution: | (HE yuan,YAN ping,HU Jia-wei,et al.Department of Forensic Medicine,School of Medical,Wuhan University,Wuhan 430071,China) |
| |
| Abstract: | Objective To explore the liver nuclear DNA degradation in rats after death by single cell gel electrophoresis and to provide a new method for the estimation of early postmortem interval.Methods A rat death model was established.The samples of liver were taken at 3-hour-interval within 30 hours after the rat's death.After single cell gel electrophoresis,the comet images were captured by the confocal microscope and analyzed by SCGE image analysis software(IMI1.0).Results An evident 'comet tail' was observed after SCGE Assay.The 'tail length' and the 'tail moment' increased with an increased PMI(0~18h),which shows strong statistical correlation with PMI.Conclusion The SCGE Assay of liver nuclear DNA degradation may be used as a predictor of early postmortem interval. |
| |
| Keywords: | Forensic pathology Postmortem interval(PMI) DNA degradation Single cell gel electrophoresis(SCGE) SCGE image analysis software(IMI1 0) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
