大鼠原发性脑干损伤早期脑干的免疫组织化学研究

用自制的簧片打击装置弹击大鼠枕骨结节，造成严重颅脑损伤或原发性脑干伤，取其脑干（中脑、桥脑及延髓）常规包埋、制片后作LSAB－FN染色。结果表明，在严重颅脑损伤及原发性脑干伤，可见FN沉积于血管周围间质、部分神经纤维内及一些神经细胞内，且随着损伤时间的延长，FN沉积量增加、范围增大。LSAB－FN法可用于生前原发性脑干伤与死后伤的鉴别和推断在3小时内损伤经过时间。

PRIMARY BRAIN-STEM INJURY STUDIED IMMUNOHISTOCHEMICALLY IN RATS

Wistar rats were hitted on their occipital protuberance by self-made ejecting instrument. Brainstems (midbrains,pons and medullary bult)of every rat were isolated,routinely embedded and sliced.And LSAB-FN stainings were performed. Results were as follows: (1)FN was observed only in arachnoid membrane, blood vessels and its endothelial cells in brain-stern in control group; (2)Distributionof FN in the postmortem injury group was the same as that in the control group; (3)In the primarybrain-stem injury group and the severe craniocerebral injury group,FN could be detected around capillaries,in between nerve fibers and some nerve cells. The amount and the extend of FN deposition increased along with the prolongation of time elapsed after injury. It was indicated that the LSAB-FNstain method. Can be applied to distinguish the antemortem primary brain-stem injury and the postmortem injury. It can also be used for timing of 3h after trauma.