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| 中国朝鲜族线粒体DNA编码区序列多态性 | |
| 引用本文： | 张永吉,李哲,徐京男,李松花,段洪瑞.中国朝鲜族线粒体DNA编码区序列多态性[J].中国法医学杂志,2011,26(2):124-126,129. |
| 作者姓名： | 张永吉 李哲 徐京男 李松花 段洪瑞 |
| 作者单位： | 1. 延边大学基础医学院,吉林,延吉,133000 2. 吉林天平司法鉴定中心,吉林,延吉,133000 3. 延边州公安局刑事科学技术研究所,吉林,延吉,133000 |
| 基金项目： | 国家自然科学基金项目(30660201) |
| 摘 要： | 目的调查中国朝鲜族群体线粒体DNA（mtDNA）编码区内5个部位 3954～4506nt、5218～5974nt、7942～871Int、10296～10653nt 及14496～14867nt的序列多态性。方法采用PCR产物直接测序方法，对212名中国朝鲜族（吉林省延边地区）无关个体进行序列多态性变化和单倍型分布调查。结果在212名无关个体中，共分出148种单倍型。遗传变异度为0．9931，耦合概率为0．0116。测序结果与Anderson标准序列比较，共检测出109个变异位点，其中79个已收录于MITOMAP。结论mtDNA编码区多态性联合应用可以提高mtDNA的个体识别能力。町为相关遗传学研究提供基础数据资料。 |
| 关 键 词： | 法医物证学 线粒体DNA编码区 单核苷酸多态性 个体识别 中国朝鲜族 |
The sequence polymorphisms of mitochondrial DNA coding region in Chinese Korean ethnic group |
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| ZHANG Yongji,LI Zhe,XU Jingnan,LI Songhua,DUAN Hongrui.The sequence polymorphisms of mitochondrial DNA coding region in Chinese Korean ethnic group[J].Chinese Journal of Forensic Medicine,2011,26(2):124-126,129. | |
| Authors: | ZHANG Yongji LI Zhe XU Jingnan LI Songhua DUAN Hongrui |
| Institution: | (ZHANG Yongji1,LI Zhe2,XU Jingnan3,LI Songhua1,DUAN Hongrui1/1.Department of Forensic Medicine,Yanbian University Collegeof Medicine,Jilin Yanji 133000,China,2.Forensic Science Center,Jilin Tianping,3.Department of Forensic Science,Yanbian States Police Station,China) |
| Abstract: | Objective To investigate the sequence polymorphisms of mtDNA coding region in Chinese Korean population.Methods Five segments of mtDNA coding region,encompassing position 3954～4506nt,5218～5974nt,7942～8711nt,10296～10653nt and 14496～14867nt,were analysis using polymerase chain reaction(PCR) and direct sequencing method from 212 unrelated Korean individuals living in Yanbian,China.Results Total 148 haplotypes were observed in the Korean population studied.The haplotype diversity and random match probability were estimated to be 0.9931 and 0.0116 respectively.Compared with the Anderson's sequence,109 sites of different nucleotide variances were found,of which 79 sites were previously registered in MITOMAP.Conclusion The results suggested that these sequence polymorphisms of mtDNA coding region are of high value in forensic application in Chinese Korean population. |
| Keywords: | forensic biological evidence mtDNA coding region Korean ethnic group single nucleotide polymorphisms identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |