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| 肿瘤组织9个STR基因座及Amelogenin基因座突变 | |
| 引用本文： | 刘开会,王占海,季安全,王建国,邱宁,王坚,王传海,郭燕霞,常彩琴,曹侠.肿瘤组织9个STR基因座及Amelogenin基因座突变[J].中国法医学杂志,2002,17(2):81-84. |
| 作者姓名： | 刘开会 王占海 季安全 王建国 邱宁 王坚 王传海 郭燕霞 常彩琴 曹侠 |
| 作者单位： | 1. 公安部物证鉴定中心,北京,100038 2. 青海省公安厅,西宁,810000 3. 青海省人民医院病理科,西宁,810000 4. 北京市朝阳公安分局,北京,100020 |
| 摘 要： | 目的 探讨CODIS系统中的9个STR基因座及Amel基因座在肿瘤组织中的变异。方法 收集20个个体的肿瘤组织及其血样,Chelex100法提取DNA,用Profiler plus系统复合扩增,310型遗传分析仪检测。结果 检验的10个基因座均有基因发生突变;20个个体肿瘤组织有6个个体的基因发生突变,变异率超过30％;同一个体的肿瘤组织发生基因突变的基因座最多为6个。结论 慎用肿瘤组织作为医疗纠纷、失踪人员的检材及对照样本。 |
| 关 键 词： | STR基因座 Amel基因座 肿瘤组织 基因突变 |
| 文章编号： | 1001-5728(2002)02-0081-04 |
| 修稿时间： | 2001年11月1日 |
A study on the gene alterations in 9 STR loci and Amelogenin locus in cancerous tissues |
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| LIU Kaihui,WANG Zhanhai,JI Anquan,et al..A study on the gene alterations in 9 STR loci and Amelogenin locus in cancerous tissues[J].Chinese Journal of Forensic Medicine,2002,17(2):81-84. | |
| Authors: | LIU Kaihui WANG Zhanhai JI Anquan |
| Institution: | LIU Kaihui,WANG Zhanhai,JI Anquan,et al. /Institute of Forensic Science,Beijing,100038 |
| Abstract: | Objective To study genetic alterations in 9 STR loci and the Amelogenin locus in various tumor tissues. Methods twenty cancer tissues taken from 20 different unrelated individuals and their blood specimens were examined with Chelex-100 extraction of DNA, Profiler Plus PCR amplification and 310 Genetic Analyzer. Results All of the 10 STR loci exist genetic alterations. The genetic alterations occurred in 6out of 20 cases. The rate of genetic alteration was 30%. Six genetic alterations were found in one tumor tissue. Conclusion The forensic community has to take be cautious not to use the tumor tissue for personnel identification. |
| Keywords: | STR loci Amelogenin locus Tumor tissues Genetic alteration |
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