用PCR—测序法对人类线粒体DNA多态区的研究

用PCR方法，对线粒体DNA多态区15997至三6401区域进行扩增，产物DNA经纯化处理局直接进行自动化序列测定，得到了准确的序列结果。将得到的中国人mtDNA多态区序列与国外已报道的相应序列进行对比，证实我们所测序列与白种人相应序列之间存在碱基差异。

A STUDY OF HUMAN MITOCHONDRIAL DNA POLYMORPHISM REGION SEQUENCING

By means of Polymerase chain reaction (PCR), the mitochondrial DNA polymorphous region from the base number 15997 to 16401 was amplified. After purification,the amplified fragment was directly Sequenced on automatic .DNA sequencer and accurate results was obtained. Compared with the former reports, there are base divergence between the results of this paper and sequence of Caucasian. Changes of base in six samples were observed.The numbers of base change are 3 to 6. The maternal inheritance of the mitochondrial genome makes mtDNA sequencing via PCR an attractive tool for testing whether individuals are maternally related. The method can also be used for studying of race inheritance and molecular evolution. The method is rather simpler and quicker than the former methods.