| FTA卡在微量血痕DNA多态性分析中的应用 |
| |
| 引用本文： | 宣金锋,丁梅,王保捷,庞灏,邢佳鑫,孟菁华.FTA卡在微量血痕DNA多态性分析中的应用[J].中国法医学杂志,2011,26(3):218-220. |
| |
| 作者姓名： | 宣金锋 丁梅 王保捷 庞灏 邢佳鑫 孟菁华 |
| |
| 作者单位： | 中国医科大学法医学院,辽宁沈阳,110001 |
| |
| 摘 要： | 目的探索FTA卡样本DNA最小检出量及同一FrA卡样本进行多项目检测的可行性。方法制作含有不同浓度DNA丌A卡，使用Identifiler试剂盒进行检验；对同一rrA卡样本先后使用Identifiler、Y-filer试剂盒进行扩增及线粒体高变区HVI区测序，使用ABl3130测序仪检测各扩增产物。结果载有0．5ngDNA的FTA卡扩增产物即可正确分型，对同一血痕样本使用不同试剂盒检验，均可清晰正确判型，线粒体高变区HVI区测序图清晰，且各检验结果均与对照一致。结论载有0．5ngDNA的FTA卡扩增产物可用于DNA分型检测，FTA卡对DNA结合较稳定，可用于多项目检测。
|
| 关 键 词： | 法医物证学 DNA多态性 FTA卡 微量DNA |
Study on DNA polymorphism in the trace bloodstain on FTA card |
| |
| XUAN Jinfeng,DING Mei,WANG Baojie,PANG Hao,XING Jiaxin,MENG Jinghua.Study on DNA polymorphism in the trace bloodstain on FTA card[J].Chinese Journal of Forensic Medicine,2011,26(3):218-220. |
| |
| Authors: | XUAN Jinfeng DING Mei WANG Baojie PANG Hao XING Jiaxin MENG Jinghua |
| |
| Institution: | XUAN Jinfeng,DING Mei,WANG Baojie,PANG Hao,XING Jiaxin,MENG Jinghua/School of Forensic Medicine,China Medical University,Shenyang 110001,China |
| |
| Abstract: | Objective To explore the minimum DNA amount for accurately genotyping on FTA card and its potentiality for effectively detecting multiple genetic markers.Methods Polymorphic analyses of the DNA samples prepared with different amounts on FTA cards were performed by using Identifiler kit.The genetic markers that may be identified by Identifiler kit,Y-filer kit and mtDNA sequencing were determined one after the one from the same sample on FTA card.Results Sample with 0.5 ng DNA on the FTA card may be accuratel... |
| |
| Keywords: | forensic biological evidence DNA polymorphism FTA card trace DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
