实验性大鼠急性心肌缺血的组织化学和扫描电镜观察

本文报告40只大鼠结扎左冠状动脉所致实验性心肌梗死早期（缺血1～8h）的病理形态学变化。结果发现苏木素-碱性复红-苦味酸（HBFP）和吖啶橙荧光染色诊断早期心肌缺血较敏感，氯化-2，3，5-三苯基四氮唑（TTc）大体染色法显示缺血境界鲜明可靠。此三种方法不受室温（15～20℃）死后自溶12～48h的影响。酸性复红染色（AF）对早期缺血组织较敏感，但受死后自溶影响，妨碍认定缺血区。扫描电镜（SEM）观察心肌缺血改变基本与透射电镜（TEM）相同，与早期自溶改变难以区别。

HISTOCHEMICAL AND SEM STUDY OF EXPERIMENTAL RATS ACUTE MI

Fourty wistar rats were used for experimental study of myocardial infarction (MI )induced by ligation of the left coronary artery. The results showed that the AF and HBFP stalng as well as Ao fluorescence were sensitive in demonstrating the early MI changes occuced 1 ~ 2 hour after coronary arteryligation. The TTC stamg method was reliable for demonstrating the MI margin. Both HBFP and AOfluorescence were under no influeence of the post-mortem autolysis, but the AF reaction were undeithe influence of the early post-mortem autolysis. Four hours were required for complete developemenof MI. The early stage of MI demonstrated by SEM was conslstant with that bv TEM. Both resuhwere influenced by the post-mortem autolysis.