| 应用斑点ELISA快速进行体液(斑)的血型测定 |
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| 引用本文： | 周斌,郭景元,汪传喜,陈建.应用斑点ELISA快速进行体液(斑)的血型测定[J].中国法医学杂志,1989(3). |
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| 作者姓名： | 周斌 郭景元 汪传喜 陈建 |
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| 作者单位： | 中山医科大学法医系，中山医科大学法医系，中山医科大学法医系，司法鉴定科学技术研究所 |
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| 摘 要： | <正> 在法医学上体液(斑)的血型测定一般常规应用中和试验、吸收试验、解离试验及混合凝集反应。这些试验既耗时,又需较高的技术,且要新鲜标准红细胞指示结果。本实验系应用我们自己提纯标酶的抗-A,-B与抗-H单克隆抗体A,利用斑点ELISA来进行体液(斑)的血型测定。通过颜色变化直接判断血型。
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THE RAPID DETERMINATION OF ABO BLOOD GROUP IN BODY FLUIDS (OR STAINS)BY DOT-ELISA USING ENZYME-LABELLED MONOCLONAL ANTIBODY |
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| Zhou Bin,Guo Jingyuan,Wang Chuanxi,Chen Jian The.THE RAPID DETERMINATION OF ABO BLOOD GROUP IN BODY FLUIDS (OR STAINS)BY DOT-ELISA USING ENZYME-LABELLED MONOCLONAL ANTIBODY[J].Chinese Journal of Forensic Medicine,1989(3). |
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| Authors: | Zhou Bin Guo Jingyuan Wang Chuanxi Chen Jian The |
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| Institution: | Zhou Bin,Guo Jingyuan,Wang Chuanxi,Chen Jian The Department of Forensic Medicine,Sun Yat-sen University of Medical Sciences,Guangazhou Institute of Forensic Siences,Ministry of Justice,Shanghai |
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| Abstract: | One step dot-ELISA method for the rapid determination of ABO blood groups in humam body fluids(or stains)was established using enzyme-labelled anti-A,--B and anti--H monoclonal antibody (McAb).The sample was applied on the nitrocellulose membrance as a dot. After washing, the appropriate McAb wasadded on top of the dot, and then followed by adding 3, 3'-diaminobenzidine(DAB). The brown color indi-cated the positive reaction. The ABO blood typing of 521 saliva samples including both secretor and non-se-cretor were carried out. All the results were correct. The advantages of this method are accurate, sensitive,rapid, easy to perform, as well as not time consuming. It is more sensitive than the conventional hemaggluti-naton inhibition test. |
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| Keywords: | ABH enzyme-labelled McAb Dot-ELISA ABO blood group Body fluid stains |
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