| 人精浆特异蛋白P_30的分离纯化与鉴定 |
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| 引用本文： | 陈东风,张琦.人精浆特异蛋白P_30的分离纯化与鉴定[J].中国法医学杂志,1987(4). |
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| 作者姓名： | 陈东风 张琦 |
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| 作者单位： | 公安部第二研究所，公安部第二研究所 |
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| 摘 要： | 本文介绍人精浆特异蛋白P_(30)分离纯化及鉴定的方法。人精浆经蒸馏水透析后经Sephadex G100、G150和G75三种不同的柱层析即可获得精浆特异蛋白。SDS-PAGE、免疫电泳、免疫双扩散证明其纯度和特异性均符合要求。它与已知抗P_(30)血清呈强阳性反应,用其免疫制备的抗血清和已知抗P_(30)血清,在免疫电泳中只与精浆或精浆特异蛋白形成一条完全相同的沉淀线,SDS-PAGE测得分子量约为30,000。故笔者将其亦定名为P_(30)。
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| 关 键 词： | 人精浆特异蛋白 P_(30) 分离鉴定 |
ISOLATION AND CHARACTERIZATION OF THE HUMAN SEMEN-SPECIFIC PROTEIN P30 |
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| Chen Dongfeng Zhang Qi Forensic Science institute,Ministry of public Security,Beijing.ISOLATION AND CHARACTERIZATION OF THE HUMAN SEMEN-SPECIFIC PROTEIN P30[J].Chinese Journal of Forensic Medicine,1987(4). |
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| Authors: | Chen Dongfeng Zhang Qi Forensic Science institute Ministry of public Security Beijing |
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| Abstract: | A method of isolation and characterization of the human semen-specific protein P_(30) is discribed in this paper, pooled human seminal plasma was dialyzed against distilled water and then applied to three columns of Sephadex G100, G150 and G75 consecutively. The isolated human semen-specific protein was characterized by SDS-PAGE, immunodiffusiou and immunoelectrophoresis. It reacted with the known anti-P_(30).Its antiserum formed the same immunoelectrophoretic patterns with the human seminal plasma as the known anti-P_(30).It has a molecular weight of about 30,000 demonstrated by SDS-PAGE. We conclude that the human semenspecific protein isolated by this method would be named as P_(30) in this report. |
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| Keywords: | Human semen-specific protein P_30 Isolation characterization |
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