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| 大鼠尸体组织中三甲胺-氮的检测 | |
| 引用本文： | 刘茜,沙滢涛,刘艳,杨帆,周兰,刘良.大鼠尸体组织中三甲胺-氮的检测[J].中国法医学杂志,2009,24(2). |
| 作者姓名： | 刘茜 沙滢涛 刘艳 杨帆 周兰 刘良 |
| 作者单位： | 1. 华中科技大学同济医学院法医学系,湖北,武汉,430030 2. 安阳市公安局,河南,安阳,455000 3. 华中科技大学同济医学院法医学系,湖北,武汉,430030;中国政法大学,证据科学教育部重点实验室,北京,100040 |
| 基金项目： | 湖北省卫生厅科研基金 |
| 摘 要： | 目的建立检测尸体组织中三甲胺-氮(TMA-N)含量的方法。方法大鼠肌肉组织样本100mg经预处理后,将三甲胺(TMA)抽提于无水甲苯中,与苦味酸作用,形成黄色的苦味酸三甲胺盐,用分光光度计在410nm处检测吸光度,标准曲线法计算TMA-N含量。结果在1～10mg/L范围内,光密度(OD)值与TMA-N浓度呈现良好线性关系(R2=0.9991,P<0.001)。平均日内差2.37%,平均日间差3.2%。加样平均回收率98.4%;大鼠死后即刻至7d,尸体肌肉组织中TMA-N含量由0.115mg/L上升至18.073mg/L,随死后时间延长呈现增加的趋势。结论死后尸体组织中的TMA-N含量可用分光光度法检测。 |
| 关 键 词： | 法医病理学 死亡时间 分光光度法 三甲胺 尸体腐败 |
Detecting the concentration of Trimethylamine-nitrogen in corpse of rat |
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| LIU Qian,SHA Yingtao,LIU Yan,YANG Fan,ZHOU Lan,LIU Liang.Detecting the concentration of Trimethylamine-nitrogen in corpse of rat[J].Chinese Journal of Forensic Medicine,2009,24(2). | |
| Authors: | LIU Qian SHA Yingtao LIU Yan YANG Fan ZHOU Lan LIU Liang |
| Institution: | LIU Qian1,SHA Yingtao2,LIU Yan1,YANG Fan1,ZHOU Lan1,LIU Liang1,3/1. Faculty of Forensic Medicine,Tongji Medical College of Huazhong University of Science , Technology,Wuhan 430030,China,2. Anyang Police Department,Henan 455000,3. China University of Political Science , Law,Ministry of Education Key Laboratory of Evidence Science,Beijing 100040 |
| Abstract: | Objective To build an experimental method for the determination of trimethylamine-nitrogen(TMA-N) in the tissue of corpse. Methods 100mg of muscle of rat corpse was preprocessed before trimethylamine was extracted with anhydro-methylbenzene, then trinitrophenol was added into extracts, and yellow compounds emerged. The optical density (OD) was detected by spectrophotometer at 410nm and the concentration of TMA-N was calculated using the standard curve. Results There was good linear correlation (R2=0.9991,P<... |
| Keywords: | forensic pathology postmortem interval spectrophotometry trimethylamine-nitrogen spoilage of corpse |
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