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| 48个X-SNP位点的筛选及法医学应用价值分析 | |
| 引用本文： | 畅晶晶,李莉,张素华.48个X-SNP位点的筛选及法医学应用价值分析[J].中国法医学杂志,2010,25(1). |
| 作者姓名： | 畅晶晶 李莉 张素华 |
| 作者单位： | 1. 复旦大学上海医学院法医学系,上海,200032;司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063 2. 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063 3. 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海,200063;苏州大学基础医学院,江苏,苏州,215123 |
| 基金项目： | 上海市自然科学基金项目(08ZR1419800);;科研院所社会公益研究专项项目(GY0805) |
| 摘 要： | 目的对筛选出的48个X-SNP位点进行遗传学分析,评价其法医学应用价值。方法根据NCBI和Hap-Map网站提供的位点信息筛选出48个高信息量X-SNP位点,利用SNPlexTMSystem技术平台进行分型检测,通过中国华东地区汉族人群200个无关个体的调查建立遗传学资料,对48个X-SNP位点的遗传多态性进行研究分析,并进行连锁不平衡检验。结果除rs6527549外,筛选出的48个X-SNP位点在中国华东汉族人群中具有高信息量,多态信息含量均在0.32以上,个体识别率在女性群体和男性群体分别为0.56和0.40以上,非父排除率在二联体和三联体中分别为0.20和0.32以上。检验发现,部分位点存在连锁不平衡现象。结论本实验选取的48个X-SNP位点分型结果稳定,重复性好,在法医生物学研究中具有较高的应用价值,适于开展大规模的高通量检测。 |
| 关 键 词： | 法医物证学 遗传多态性 SNPlex 单核苷酸多态性 连锁不平衡 |
Selection and evaluation of forensic application value of 48 X-SNP loci |
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| CHANG Jingjing,LI Li,ZHANG Suhua.Selection and evaluation of forensic application value of 48 X-SNP loci[J].Chinese Journal of Forensic Medicine,2010,25(1). | |
| Authors: | CHANG Jingjing LI Li ZHANG Suhua |
| Institution: | CHANG Jingjing 1;2;LI Li2;ZHANG Suhua 2;3/1.Department of Forensic Medicine;Shanghai Medical College;Fudan University;Shanghai 200032;China;2.Shanghai Key Laboratory of Forensic Medicine;Institute of Forensic Science;Ministry of Justice;P.R.China;Shanghai 200063;3.Medical School of Soochow University;Jiangsu Suzhou 215123;China |
| Abstract: | Objective To analyze and evaluate the 48 selected highly polymorphic SNP loci in Chinese Han population on genetic and forensic aspects.Methods Samples from 200 unrelated Han individuals in East China were collected.48 X-SNP polymorphic genetic markers were selected according to the information provided by NCBI and HapMap,and then were typed by SNPlexTMSystem for establishing genetic data.Results The 48 X-SNP loci were highly polymorphic markers in East China Han population except rs6527549,and their polymo... |
| Keywords: | forensic biology genetic polymorphism SNPlex single nucleotide polymorphism linkage disequilibrium |
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