| 降解检材STR分型Ladder-Like条带形成原因的探讨 |
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| 引用本文： | 楼迪栋,余纯应,黄代新,赵贵森,任文,杨庆恩.降解检材STR分型Ladder-Like条带形成原因的探讨[J].中国法医学杂志,2005,20(6):332-334. |
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| 作者姓名： | 楼迪栋 余纯应 黄代新 赵贵森 任文 杨庆恩 |
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| 作者单位： | 1. 浙江省温州医学院法医学系,浙江,温州,325000;华中科技大学同济医学院法医系,湖北,武汉,430030
2. 华中科技大学同济医学院法医系,湖北,武汉,430030 |
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| 摘 要： | 目的探讨降解DNA短串联重复序列PCR扩增产物在PAGE中Ladder-L ike条带的形成原因。方法用一组人工合成的不同长度的D8S1179DNA单链,模拟降解检材中的断裂DNA;利用John M设计的D8S1179引物对模拟模板的DNA单链进行PCR扩增,PAGE分离。结果不同模拟模板的DNA单链的组合可以扩增出不同的Lad-der-L ike条带。结论短串联重复序列Ladder-like条带是PCR过程中降解DNA多态区互补扩增的产物。
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| 关 键 词： | 法医物证学 短串联重复序(STR) Ladder-like条带 PCR-STR分型降解检材 |
| 文章编号： | 1001-5728(2005)06-0332-03 |
| 修稿时间： | 2004年12月7日 |
The cause of Ladder-like bands in the STR profiling of the highly degraded remains |
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| Institution: | ( Lou Di-dong,Yu Chuen-ying,Huang Dai-xin,et alDepartment of forensic Medicine,Huazhong University,Wuhan 430030,China) |
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| Abstract: | Objective To elucidate the mechanism of Ladderlike bands in the PCR-STR profiling of the high-degraded remains.Methods Some ssDNA of D8S1179 with one flanking sequence and several tandem repeats similar to randomly degraded DNA in decomposed remains were synthesized.The mixtures including diverse synthesized DNA possessing several repeats which can in some instances anneal complementary tandem repeat stretches are performed by PCR.Results artifact ladder is detected on PAGE.Conclusion Mimicking the components of degraded remains,in this study,we demonstrate that it is the main cause of Ladder-like artifact from decomposed remains to anneal at complementary tandem repeat in a 4bp phasing manner. |
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| Keywords: | Forensic biolgkol evidence Short tandem repeats Ladder-like bands PCR-STR profiling Highly-degraded remains |
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