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| 单细胞检验技术用于混合上皮细胞的分离和检验 | |
| 引用本文： | 黄江平,李彩霞,亓冰,任文彦,胡兰.单细胞检验技术用于混合上皮细胞的分离和检验[J].中国法医学杂志,2011,26(1):39-41. |
| 作者姓名： | 黄江平 李彩霞 亓冰 任文彦 胡兰 |
| 作者单位： | 1. 中国人民公安大学,北京,100038;上海市公安局物证鉴定中心,上海,200083 2. 公安部物证鉴定中心,北京,100038 3. 南京市公安局浦口分局,江苏,南京,211800 4. 中国人民公安大学,北京,100038 |
| 基金项目： | 国家“十一五”科技支撑计划项目(2006BAK07B01) |
| 摘 要： | 目的建立单细胞显微捕获联合低体积扩增技术,用于混合上皮细胞检材分离检验。方法取5名男性口腔上皮细胞拭子浸泡液30μL,分别滴加到5份含同一女性皮肤表皮细胞拭子上,制成5份混合上皮细胞样本为实验组,同时制备同样的5份样本为对照组。实验组样本采用显微捕获单个口腔上皮细胞,并使用低体积扩增技术进行扩增;对照组用M48纯化试剂盒提取DNA,Identifiler试剂盒复合扩增,扩增体系为10μL。所有产物均用ABI 3130遗传分析仪进行STR分型。结果 5份实验组样本均得到男性STR分型结果,5份对照组样本则均仅得到混合分型结果。将该方法应用于1例强奸杀人案例检验,取得了满意效果。结论单细胞显微捕获联合低体积扩增技术可用于混合上皮细胞样本的分离检验。 |
| 关 键 词： | 法医物证学 单细胞检验技术 混合上皮细胞检材 |
Isolation and identification of buccal cells from mixed epithelial cell samples using single cell separation technique |
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| HUANG Jiangping,LI Caixia,QI Bing,REN Wenyan,HU Lan.Isolation and identification of buccal cells from mixed epithelial cell samples using single cell separation technique[J].Chinese Journal of Forensic Medicine,2011,26(1):39-41. | |
| Authors: | HUANG Jiangping LI Caixia QI Bing REN Wenyan HU Lan |
| Institution: | (HUANG Jiangping1,4,LI Caixia2,QI Bing3,REN Wenyan1,HU Lan2 1.Chinese People's Public Uninversity,Beijing 100038,China,2.Institute of Forensic Science of Ministry of Public Security,3.Pukou District Public Security Sub-bureau,Nanjing 211800,4.Institute of Forensic Science,Shanghai Public Security Bureau,Shanghai 200083,China) |
| Abstract: | Objective To developed a technique combining micromanipulation with on chip LV-PCR system to enable the isolation and identification of buccal cells from biological mixtures.Methods Mock mixture samples,prepared with male oral epithelial cells and female skin epidermal cells,were detected by single cell separation technique,and conventional method in parallel.The conventional method was conducted as follows: cell DNA were extracted by M48 purification kit and then a 10 mL-volume PCR was performed.Amplified ... |
| Keywords: | forensic biological evidence single cell separation method mixed epithelial cell samples |
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