新型微流体液相色谱(micro-LC)串联四极杆质谱检测头发中5种苯二氮卓类药物

目的建立了新型微流体液相色谱(micro-LC)串联四级杆质谱检测头发中5种苯二氮卓类药物(三唑仑,α-羟基咪达唑仑,咪达唑仑,α-羟基阿普唑仑,普拉西泮)。方法采用液-液萃取法(LLE)提取毛发样品,微流体液相色谱串联四级杆质谱检测。结果在回归系数大于0.99的情况下,目标分析物的良好线性。精密度和萃取效率分别在2.7%~17.1%和66.5%~120.5%之间。每个分析物的检出限和定量限分别为0.001~0.08 pg·mg^-1和0.0125~0.25pg·mg^-1。结论本方法与传统的液相色谱串联质谱法(LC/MS-MS)相比灵敏度出高2-10倍。本研究展示了新型微流体分离系统串联质谱方法的实用性,并为法医毒理学领域关于头发样本中痕量药物检测提供了一种新的检测手段。

Ultrasensitive analysis of 5 benzodiazepines in hair samples using microfluidic tile technology and LC-MS/MS

Objective To establish a novel microfluidic LC-MS/MS method for the analysis of 5 benzodiazepines(triazolam,α-hydromidazolam,midazolam,α-hydroalprazolam,prazepam)in hair samples.Methods Samples were extracted with liquid-liquid extraction procedure and analyzed by microfluidic LC-MS/MS method.Results A good linearity for the target analytes was measured with regression coefficients above 0.99.Precisions and extraction efficiencies were in the range of 2.7%-17.1% and 66.5%-120.5%,respectively.LODs and LOQs of each analyte ranged from 0.001 to 0.08 pg·mg^-1 and 0.0125 to 0.25 pg·mg^-1,respectively.Conclusion All of the analytes display 2-10 folds higher LODs by the novel microfluidic LC-MS/MS method as compared to values obtained by the traditional LC-MS/MS method.A dramatic increase in sensitivity was achieved as compared to published literatures.The proposed method was successfully applied to real hair samples from a drug consumer and satisfactory result was obtained.The present study demonstrates the utility of the novel microfluidic LC-MS/MS method as well as provides an alternative strategy for forensic toxicological analysis of trace drug testing in hair.