| 固相萃取-气相色谱-质谱法检验人血浆中的右美托咪定 |
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| 引用本文： | 王瑞花,刘锐,栾玉静,董颖,任昕昕.固相萃取-气相色谱-质谱法检验人血浆中的右美托咪定[J].中国法医学杂志,2017(5):497-499. |
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| 作者姓名： | 王瑞花 刘锐 栾玉静 董颖 任昕昕 |
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| 作者单位： | 1. 公安部物证鉴定中心,北京,100038;2. 河北省公安厅,河北 石家庄,050092 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项基金计划项目"体液中丙泊酚、甲苯噻嗪、右美托咪定三种快速麻醉药的检验方法研究预言"(2013JBYY001) |
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| 摘 要： | 目的采用固相萃取结合气相色谱-质谱法(SPE-GC/MS)检验人血浆中盐酸右美托咪定。方法采用SPE提取血浆,用GC-MS/MS方法测定。结果盐酸右美托咪定在0.2μg/m L~5.0μg/m L范围内与峰面积呈现良好的线性关系(r2=0.999 1),检出限为20.0ng/m L,回收率为86.1%~91.5%,日内日间精密度均小于7.86%。结论本方法操作简单,结果准确,可以作为测定人血浆中右美托咪定的方法。
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| 关 键 词： | 法医毒物分析 右美托咪定 血浆 固相萃取 气相色谱-质谱 |
Determination of dexmedetomidine in human plasma by solid phase extraction with gas chromatography-mass spectrometry |
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| Wang Ruihua,Liu Rui,Luan Yujing,Dong Ying,Ren Xinxin.Determination of dexmedetomidine in human plasma by solid phase extraction with gas chromatography-mass spectrometry[J].Chinese Journal of Forensic Medicine,2017(5):497-499. |
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| Authors: | Wang Ruihua Liu Rui Luan Yujing Dong Ying Ren Xinxin |
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| Abstract: | Objective To develop the analytical method of dexmedetomidine in human plasma by solid phase extraction with gas chromatography-mass spectrometry(SPE-GC/ MS). Methods The human plasma were extracted with a solid phase extraction(SPE) and determined by GC/MS. Results The lowest detectable limit was 0.05μg/mL, the standard curve was linear over the range of 0.2μg/mL~5μg/mL. The absolute recovery was 86.1%~91.5%. The intra-and interday precision was within 7.86% at three concentrations. Conclusion Since the procedure proved to be simple, quick and sensitive, it could be used for the determination of dexmedetomidine in human plasma. |
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| Keywords: | forensic toxicological analysos dexmedetomidine plasm solid phase extraction gas chromatography-mass spectrometry |
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