刑事现场188份接触DNA检材法医学检验分析

目的分析188份接触DNA检材的提取、送检和检验结果,探讨接触DNA检出率及可能影响接触DNA检验的因素。方法收集本辖区2016年1月至2016年10月提取并送检的188份接触DNA检材,按照检材载体性质、提取方法、送检时间、检出率等进行分类,采用SPSS13.0软件对数据进行统计分析和χ2检验。结果188份接触DNA检材成功进行STR分型的有38份,检出率为20.21%;其中表面质软、粗糙的载体接触DNA检出率58.82%,高于其它载体接触DNA检出率组的差异具有统计学意义;直接原物提取的接触DNA检材检出率42.11%,高于脱落细胞粘取器提取、棉签拭子转移提取的检出率组的差异具有统计学意义;送检时间早的检材检出率高于送检时间晚的检材组且具有统计学意义。结论接触DNA检材的检出率受载体性质、提取方法、送检时间等因素影响,日常现场勘查时要注重发现检出率高的载体上的接触DNA选择适当的方法提取,并及时送检。

Inspection and analysis of 188 contact DNA samples

Objective To analyze the detection rate of contact DNA samples and the possible influencing factors of contact DNA test. Methods 188 contact DNA samples collection method of the area in January 2016 to October 2016 and from submission to the laboratory, in accordance with the vector properties, extraction method, inspection time and detection rate of classification for statistical analysis. Results All 188 samples were detected by STR genotyping,and 38 samples genotyped DNA successfully. The detection rate was 20.21%; the surface of soft, coarse carrier of the contact detection rate of DNA was higher than the carrier surface smooth and hard contact; DNA samples extracted from the original direct detection rate higher than the detection rate of exfoliated cells from sticky extraction; contact DNA samples inspection time more early detection rate is higher than that of the late submission time. Conclusion DNA samples the detection rate by carrier properties, extraction methods, inspection time and other factors, should pay attention to the daily scene found in the detection of DNA carrier rate on the contact and extraction, using proper method timely submission of investigation.