| 荧光标记3个miniSTR基因座复合扩增系统的建立 |
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| 引用本文： | 王会品,刘超,陈晓晖,刘宏,刘长晖.荧光标记3个miniSTR基因座复合扩增系统的建立[J].中国法医学杂志,2007,22(5):308-310. |
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| 作者姓名： | 王会品 刘超 陈晓晖 刘宏 刘长晖 |
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| 作者单位： | 1. 中山大学中山医学院法医学系,广东,广州,510080;中山市公安局,广东,中山,528403
2. 广州市公安局刑科所,广东,广州,510030 |
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| 基金项目： | 国家自然科学基金;广东省广州市科技攻关项目 |
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| 摘 要： | 目的建立扩增片段<135bp,包括D5S818,D8S1179,D16S539 3个miniSTR基因座复合扩增系统。方法采用不同荧光染料标记引物,通过PCR扩增,利用ABI 3100遗传分析仪进行片段长度分析,对100份无关个体血样,10个家系样本以及30份高度降解检材进行检测。结果本系统DNA分型结果与AmpFLSTR Identifiler试剂盒完全一致,且灵敏度高于AmpFLSTR Identifiler试剂盒。结论本系统可以应用于个人识别和亲权鉴定,为降解DNA样本分型提供了新的方法。
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| 关 键 词： | 法医物证学 miniSTR 复合扩增 D5S818 D8S1179 D16S539 |
| 文章编号： | 1001-5728(2007)05-0308-03 |
The establishment of fluorescent multiplex set for three miniSTR loci |
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| WANG Hui-pin,LIU Chao,CHEN Xiao-hui,et al..The establishment of fluorescent multiplex set for three miniSTR loci[J].Chinese Journal of Forensic Medicine,2007,22(5):308-310. |
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| Authors: | WANG Hui-pin LIU Chao CHEN Xiao-hui |
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| Institution: | WANG Hui-pin,LIU Chao,CHEN Xiao-hui,et al.Department of Forensic Biology,Sun Yat-sen University,Guangzhou 510080,China |
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| Abstract: | Objective To establish a miniSTR multiplex set which includes three STR loci D5S818,D8S1179 and D16S539,whose amplified fragments shorter than 135bp.Methods After amplification with fluorescence labeled primers,the amplified products from 100 unrelated individuals,10 genealogies,as well as 30 highly degraded specimens,were analyzed by ABI 3100 Genetic Analyzer.The genotypes obtained by the miniSTR multiplex set were consistent with that by the AmpFLSTR Identifiler kit,and the former had a higher sensitivity than the latter.Conclusion The miniSTR multiplex set is useful in personal identification and paternity test,especially in the analysis of degraded DNA sample. |
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| Keywords: | miniSTR D5S818 D8S1179 D16S539 |
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