| 抗人N单克隆抗体的制备及其特性的研究 |
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| 引用本文： | 张秀梅,张建,段薇,郭阳,傅百川.抗人N单克隆抗体的制备及其特性的研究[J].中国法医学杂志,1987(2). |
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| 作者姓名： | 张秀梅 张建 段薇 郭阳 傅百川 |
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| 作者单位： | 辽宁省基础医学研究所，辽宁省基础医学研究所，辽宁省基础医学研究所，辽宁省基础医学研究所，辽宁省基础医学研究所 |
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| 摘 要： | 用ON型人红细胞免疫BALB/c小鼠脾细胞与SP2/O骨髓瘤细胞融合,获得分泌IgM类抗人N单克隆抗体杂交瘤细胞株(ON_1D_3)。其培养上清液抗体效价为128～256,诱生小鼠腹水的抗体效价为8192;与Panel A红细胞反应呈抗N特异性;检测273名成人红细胞,只特异性凝集N(?)MN型人红细胞,不凝集M型红细胞。与猴等9种动物红细胞无交叉反应;酶修饰试验结果表明,ON_1D_3识别的抗原决定簇是唾液酸依赖性的。
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| 关 键 词： | 抗N单克隆抗体 MN血型 |
PRODUCTION AND CHARACTERIZATION OF MOUSE MONOCLONAL ANTI-N |
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| Zhang Xiumei,Zhang Jian,Duan Wei,Guo Yang,Fu Baichuan Basic Medical Institute of Liaoning Province,Shenyang.PRODUCTION AND CHARACTERIZATION OF MOUSE MONOCLONAL ANTI-N[J].Chinese Journal of Forensic Medicine,1987(2). |
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| Authors: | Zhang Xiumei Zhang Jian Duan Wei Guo Yang Fu Baichuan Basic Medical Institute of Liaoning Province Shenyang |
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| Abstract: | BALB/c mice were immunized with human erythrocytes from homozygous group N individuals.lmmune spleen cells were fused with murine myeloma cells SP 2/0.One clone ON_1D_3 secreting monoclonal anti-N was identified. The antibody was of IgM class and specifically reacted with human group N red blood cells.In typing 273 erythrocyte samples,monoclonal anti-N reagents gave identical results with commercial anti-N typing sera of rabbit origin. Monoclonal anti-N didn't cross-react with animal red blood cells.Treatment of red cells with neuraminidase,which removes N-acetylneuraminic acid from glycophorin A,abolished ability of binding with monoclonal anti-N antibodies. It suggests that the epitope of red blood cells recognized by ON_1D_3 secreting monoclonal anti-N was sialic acid dependent. |
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| Keywords: | monoclonal anti-N antibodies MN blood type |
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