| 根据Y-染色体特异DNA序列鉴定牙齿性别 |
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| 引用本文： | 姜先华,王国林,韩景松,和中年.根据Y-染色体特异DNA序列鉴定牙齿性别[J].中国法医学杂志,1990(3). |
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| 作者姓名： | 姜先华 王国林 韩景松 和中年 |
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| 作者单位： | 辽宁省刑事科学技术研究所，辽宁省刑事科学技术研究所，辽宁省刑事科学技术研究所，辽宁省刑事科学技术研究所 |
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| 摘 要： | 人类牙齿的性别鉴定,是法医学个体识别中的一个重要部分。作者们应用限制性内切酶 HaeⅢ、Sau3A 水解60颗牙髓 DNA,琼脂糖凝胶电泳分离酶解片段,在紫外灯下直接观察。根据 Y-染色体特异 DNA 序列,3.4kb 长片段的有无,判断牙齿的性别。为牙齿性别的法医学鉴定提供了一种简单、准确的新方法。
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| 关 键 词： | Y-染色体特异 DNA 序列 荧光区带 牙髓 |
SEX IDENTIFICATION OF THE DENTAL PULP BY DETECTING Y-CHROMOSOME SPECIFIC DNA SEQUENCE |
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| Jiang Xianhua Wang Guolin Han Jinsong He Zhongnian Liaoning Criminal Scientific and Technical Research Institute,Sheuyaug.SEX IDENTIFICATION OF THE DENTAL PULP BY DETECTING Y-CHROMOSOME SPECIFIC DNA SEQUENCE[J].Chinese Journal of Forensic Medicine,1990(3). |
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| Authors: | Jiang Xianhua Wang Guolin Han Jinsong He Zhongnian Liaoning Criminal Scientific and Technical Research Institute Sheuyaug |
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| Abstract: | Sex determination of human tooth is one of the most important parts in theindividual identification in forensic medicine.The dental pulp DNA Was dige-sted with restriction donuclease and electrophoresed on agarose gel.The sexwas determined according to whether the Y-chromosome specific DNA sepuence(3.4kb long fragment)existed or not by directly observing UV light.It prov-ides a new simple method in the individual identification in forensic medicine. |
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| Keywords: | Y-chromosme specific DNA sequence Flouresence band Dental pulp |
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