人和动物SON基因3’非编码区的PCR测序分析

目的 研究人和猕猴、阿拉伯狒狒、猪、牛、羊、马、驴、骡、狗、猫、兔、大白鼠、小白鼠、豚鼠等14种哺乳类动物SON基因3’非编码区(3’UTR)的种属特异性碱基序列差异。方法 对人和上述14种哺乳类的SON基因3’非编码区中的部分种属特异性区域进行PCR测序分析。结果 人有2条扩增片段和14种哺乳类动物各有1条扩增片段的碱基序列;人无关个体和同一种属不同个体动物DNA扩增片段的碱基序列相同,人、猕猴、阿拉伯狒狒、猪、牛、羊、马、驴、骡、狗、猫、兔、大白鼠、小白鼠、豚鼠DNA的SON基因3’UTR扩增片段之间存在碱基序列差异。结论 SON基因3’UTR是一个具有良好种属特异性的遗传标记,采用SON基因3’UTR扩增测序技术可以对人和上述14种哺乳类动物进行准确的种属鉴定。

PCR sequence analysis of the 3'UTR of the SON gene of human and mammalian

Objective To study the specific sequences in the 3'untranslated region (UTR) of the SON gene of human beings and 14 kinds of mammalian. Method The specific sequences in the 3'untranslated region (UTR) of the SON gene of human beings and 14 kinds of mammalians were amplified and directly se-quenced. The sequence data of PCR products of human beings and mammalians were declared. Results The sequences of 3'UTR of SON gene of human beings differed from 14 kinds of mammalians. The sequences of samples came from different human individuals did not show any difference. Conclusion The 3'UTR of SON gene is a good DNA region for specie identification. Using this technology, DNA of human beings can be distinguished from 14 kinds of mammalians. This technology is very useful in of forensic science.