| 二阶导数光谱双波长法测定血中碳氧血红蛋白饱和度的研究 |
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| 引用本文： | 张喜轩,范垂昌,于舰.二阶导数光谱双波长法测定血中碳氧血红蛋白饱和度的研究[J].中国法医学杂志,1991(4). |
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| 作者姓名： | 张喜轩 范垂昌 于舰 |
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| 作者单位： | 中国医科大学法医化学教研室 沈阳110001 |
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| 摘 要： | 本文将双波长法用于二阶导数光谱中,提出一种新的COHb饱和度测定方法。用本法测定了已知COHb饱和度的标准血样和未知COHb饱和度的检血,都得到了比较满意的结果。
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| 关 键 词： | COHb饱和度 CO中毒 二阶导数光谱 双波长法 |
DETERMINATION OF CARBOXYHEMOGLOBIN IN BLOOD BY TWO-WAVELENGTH SECOND-DERIVATIVE SPECTROPHOTOMETRY |
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| zhang xixuan,Fan Chuichang,Yu Jian.DETERMINATION OF CARBOXYHEMOGLOBIN IN BLOOD BY TWO-WAVELENGTH SECOND-DERIVATIVE SPECTROPHOTOMETRY[J].Chinese Journal of Forensic Medicine,1991(4). |
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| Authors: | zhang xixuan Fan Chuichang Yu Jian |
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| Institution: | zhang xixuan,Fan Chuichang,Yu Jian Department of Forensic Chemistry,China Medical University,Shenyang 110001 |
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| Abstract: | A new method of determination of COHb is reported in this paper by two- wavelength second-derivative spectrophotometry.Blood was diluted with a solution of THAM containing Na_2S_2O_4.The second-derivative spectrum was measured in the region of 500-600 nm and the derivative absorbance differerce ΔAx was deter mined at the wavelengths of 564 nm and 576 nm.Then the solution was satura- ted with CO gas.The second-derivative spectrum of COHb was measured in the same region.The derivative absorbance difference ΔA_(100) was determined at the same wavelengths.The COHb in blood was calculated by the ratio of ΔAx to ΔA_(100).The results of determination of COHb in standard blood samples were in good agreement with the theoretical values. |
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| Keywords: | COHb concentration CO poisoning Second-derivative spectrum Two-wavelength |
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