| 30个 InDels在中国三个民族的遗传学调查及法医学应用 |
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| 引用本文： | 秦翠娇,迪力夏提·塔什,贾竟,董会,魏以梁,胡兰,李彩霞.30个 InDels在中国三个民族的遗传学调查及法医学应用[J].中国法医学杂志,2014(4):299-303. |
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| 作者姓名： | 秦翠娇 迪力夏提·塔什 贾竟 董会 魏以梁 胡兰 李彩霞 |
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| 作者单位： | 公安部物证鉴定中心法医遗传学公安部重点实验室;中国人民公安大学;克孜勒苏柯尔克孜自治州公安局;重庆医科大学;南方医科大学; |
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| 基金项目： | 国家自然科学基金资助项目(81202384);“十二五”国家科技支撑计划项目(2012BAK02B01) |
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| 摘 要： | 目的调查Qiagen Investigator@ DIPplex试剂盒30个InDels多态性位点在中国汉族、藏族、维吾尔族人群中的群体遗传学数据，评估其法医学应用价值。方法采集汉、藏、维吾尔族各90名无关个体静脉血，提取DNA。使用3130xL毛细管电泳对该270份样品进行分型，通过统计计算相关的群体遗传学参数。结果实验得到270份样品的分型及基因型频率，30个InDels未明显偏离Hardy-Weinberg平衡及连锁平衡，在汉族、藏族、维吾尔族三个人群中的随机匹配概率分别为1.42×10（-11）、7.19×10（-12）、4.74×10（-13），累积非父排除率（CPE）均大于0．9951。结论该组插入缺失位点在中国的汉族、藏族、维吾尔族人群中具有较高的多态性，能达到较高的个体识别能力，可以作为现有STR检验体系的补充。
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| 关 键 词： | 法医遗传学 插入缺失多态性 个体识别 汉族 藏族 维吾尔族 中国 |
Forensic genetics study of 30 InDel markers for forensic identification in three Chinese populations |
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| Institution: | Qin Cuijiao, Dilixiati · Taxi, Jia Jing, Dong Hui, Wei Yiliang, Hu Lan, Li Caixia(1. Institute of Forensic Science, Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing 100038, China ; 2. Chinese People＇s Public Security University, Beifing 100038, China; 3. Kizilsu Kirgiz Autonomous Pre- fecture in Xinjiang Public Security Bureau, Kizilsu Kirgiz Autonomous Prefecture 843500, China ; 4. Chong qing Medical University, Chongqing 400016, China; 5. Southern Medical University, Guang Zhou 510000. China) |
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| Abstract: | Objective To study 30 InDel polymorphisms （ Qiagen Investigator DIPplex kit） of three populations in China （Han, Tibetan, Uyghur） and evaluate its forensic application. Methods DNA sam- ples were extracted from Han （ n = 90）, Tibetan （ n = 90）, Uyghur （ n = 90） populations in China, genotype data of 30 InDels were obtained by 3130xl Genetic Analyzer, and population genetic parameters were calcu- lated. Results None of 30 InDels deviated from Hardy-Weinberg equilibrium and linkage equilibrium. The random match probabilities were 1.42 x 10 -1l, 7.19 x 10 -l2, 4.74 x 10 -13 for Han, Tibetan, Uyghur, re- spectively. The cumulative probabilities of exclusion （CPE） were greater than 0. 995 1. Conclusion This panel of InDels is highly polymorphic in three Chinese populations and can be served as a supplementary to the current STR system for individual identification. |
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| Keywords: | forencsic genetics InDels individual identification Han Tibetan Uyghur China |
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