| SPE-GC／MS检测生物样品中利多卡因、罗哌卡因、布比卡因 |
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| 引用本文： | 张蕾萍,何洪源,于忠山,何毅,王春媛.SPE-GC／MS检测生物样品中利多卡因、罗哌卡因、布比卡因[J].中国法医学杂志,2014(4):360-362. |
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| 作者姓名： | 张蕾萍 何洪源 于忠山 何毅 王春媛 |
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| 作者单位： | 公安部物证鉴定中心;中国人民公安大学;中国医科大学; |
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| 摘 要： | 目的建立生物样品中利多卡因、罗哌卡因、布比卡因的固相萃取提取方法和气质检测方法。方法采用Oasis HLB固相萃取柱，以GC／MS定性定量。结果生物样品中利多卡因、罗哌卡因、布比卡因平均萃取回收率达74％以上，检测限0．01μg／mL，线性关系良好，相关系数R2=0．9900以上。结论方法操作简便快速，萃取回收率高，重现性好，可用于实际案件。
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| 关 键 词： | 法医毒物分析 利多卡因 罗哌卡因 布比卡因 固相萃取 气相色谱-质谱 |
Determination of lidocaine,ropivacaine, bupivacaine in biological samples by solid phase extraction- GC/MS method |
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| Institution: | Zhang Leiping , He Hongyuan, Yu Zhongshan , He Yi, Wang Chunyuan(1. Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; 2. Chinese People＇ s Public Security University, Beijing 100038, China; 3. China Medical University, Shenyang 110001, China) |
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| Abstract: | Objective To develop a solid phase extraction method for determination of lidocaine, ropiv- acaine,bupivacaine in biological samples. Methods The sample was purified by using Oasis HLB sohd phase extraction column, and quantitatively detected and qualitatively analyzed by gas chromatography-mass spectrometry （GC/MS）. Results The mean recovery rate of lidocaine, ropivacaine, bupivacaine in biological samples was over 74%, the limits of detection was 0.01 μg/mL. The method showed excellent linearity and the linear correlation coefficient was over 0. 9900. Conclusion It can be concluded that the method may be widely used in forensic toxicological analyses, due to its simple and quick operation, high recovery rate, good repetition, and clean extraction. |
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| Keywords: | forensic toxicological analysis lidocaine ropivacaine bupivacaine solid phase extraction ： GC/MS |
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