| GC/MS、GC/NPD法检测血液中氯胺酮 |
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| 引用本文： | 邸玉敏,朱军,常靖,崔巍,于忠山.GC/MS、GC/NPD法检测血液中氯胺酮[J].中国法医学杂志,2010,25(3):169-171. |
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| 作者姓名： | 邸玉敏 朱军 常靖 崔巍 于忠山 |
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| 作者单位： | 1. 中国人民公安大学,北京,100038
2. 公安部物证鉴定中心,北京,100038 |
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| 基金项目： | 十一·五国家科技支撑计划项目 |
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| 摘 要： | 目的利用GC/MS、GC/NPD与固相萃取（SPE）技术相结合,建立血液中氯胺酮的定性定量分析方法。方法选择4-苯基丁胺为内标,采用Bond-Elut Certify固相柱萃取、二氯甲烷：异丙醇：氨水（78∶20∶2,v/v/v）洗脱的固相萃取分离技术,比较不同pH体系、洗脱溶剂对回收率的影响,建立血液中氯胺酮的GC/MS、GC/NPD定性定量分析方法。结果以GC/NPD分析氯胺酮在6.0～5000ng/mL范围内线性关系良好,GC/MS-Scan定性检测限为20.0ng/mL。方法平均回收率达96.9%,标准偏差小于5%。结论此方法可满足氯胺酮毒品滥用者血液定性定量分析。
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| 关 键 词： | 法医毒物分析 氯胺酮 固相萃取 GC/MS GC/NPD |
Determination of ketamine in blood using gas chromatography/mass spectrometry(GC/MS) and gas chromatography/nitrogen phosphor detector (GC/NPD) |
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| DI Yumin,ZHU Jun,CHANG Jing,CUI Wei,YU Zhongshan.Determination of ketamine in blood using gas chromatography/mass spectrometry(GC/MS) and gas chromatography/nitrogen phosphor detector (GC/NPD)[J].Chinese Journal of Forensic Medicine,2010,25(3):169-171. |
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| Authors: | DI Yumin ZHU Jun CHANG Jing CUI Wei YU Zhongshan |
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| Institution: | (DI Yumin1,ZHU Jun2,CHANG Jing2,CUI Wei2,YU Zhongshan2 /1.Chinese Peoples,Public Security University,Beijing 100038,China,2. Institute of Forensic Science Ministry of Public Security,China) |
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| Abstract: | Objective To develop a qualitative and quantitative analysis method for the determination of ketamine in blood samples using solid-phase extraction combined with GC/MS and GC/NPD. Methods Using 4-PBA as the internal standard,and the samples were extracted by solid-phase extraction with Bond-Elut Certify cartridge. Analytes were eluted by dichloromethane :isopropanol :ammonium hydroxide (78:20:2,v/v/v). The optimum conditions for extraction,such as pH and eluent were studied in detail. Abuse drugs extracted ... |
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| Keywords: | GC/MS GC/NPD |
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