首页 | 本学科首页   官方微博 | 高级检索  
     检索      

氯胺酮、甲基苯丙胺和吗啡金标单抗试剂盒的研制
引用本文:曾立波,CHEN Lian-kang,胡小龙,周华山,骆华,张玉荣,张润生,沈思.氯胺酮、甲基苯丙胺和吗啡金标单抗试剂盒的研制[J].中国法医学杂志,2008,23(4):241-243.
作者姓名:曾立波  CHEN Lian-kang  胡小龙  周华山  骆华  张玉荣  张润生  沈思
作者单位:上海市刑事科学技术研究所,上海,200083
基金项目:"十一五"国家科技支撑计划项目
摘    要:目的建立同步检测氯胺酮、甲基苯丙胺和吗啡的方法。方法将胶体金标记的抗氯胺酮、抗甲基苯丙胺和抗吗啡单克隆抗体浸涂在玻璃纤维膜上,将氯胺酮、甲基苯丙胺和吗啡的完全抗原以及羊抗鼠多克隆抗体喷涂在硝酸纤维素膜上,分别标定为检测区(T)和质控区(C)。样本中游离的氯胺酮、甲基苯丙胺和吗啡分别与包被的完全抗原免疫竞争结合胶体金标记抗氯胺酮、抗甲基苯丙胺和抗吗啡单克隆抗体。以质控区和检测区是否出现紫红色条带判读结果。结果对66种药品和毒品的特异性测试表明,该试剂盒仅识别氯胺酮及其代谢物、甲基苯丙胺及其衍生物和吗啡类;对人体尿样中的氯胺酮、甲基苯丙胺和吗啡检测阈值分别为1000ng/ml、1000ng/ml和300ng/ml;与GC/MS对照试验结果一致;试剂盒稳定性较好,在常温下可较长时间保存。结论本文研制的试剂盒可用于样本中氯胺酮、甲基苯丙胺和吗啡成分定性的同步检测。

关 键 词:法医毒物分析  氯胺酮  甲基苯丙胺  吗啡  胶体金  单克隆抗体

Development of colloidal gold labeled monoclonal antibody kit for simultaneous detectiion of ketamine,methamphetamine and morphine
Institution:(ZENG Li-bo,CHEN Lian-kang,HU Xiao-long,et al./Shanghai Institute of Forensic Science,Shanghai 200083,China)
Abstract:Objective To develop a convenient and rapid method for detecting ketamine,methamphetamine and morphine simultaneously.Methods Anti-ketamine,anti-methamphetamine and anti-morphine monoclonal antibodies were conjugated with colloidal gold particles separately and dispensed on the glass fiber membrane.The complete antigen ketamine-BSA,methamphetamine-BSA and morphine-BSA,and the goat anti-mouse IgG polyclonal antibody were coated on the nitrocellulose membrane as the test lines(T line) and the control line(C line).Different intensities of the purple lines on the strip could be seen as a result of the competition for binding to the conjugated antibodies between the complete antigens and free ketamine,methamphetamine and morphine which maybe present in the samples.Results Data from specificity test to 66 kinds of drug showing the test can detect ketamine,methamphetamine and morphine without any cross reactions to other kind of drugs,The cut-off value was 1 000ng/ml,1 000ng/ml and 300ng/ml for ketamine,methamphetamine and morphine separately,and got the same results with GC/MS methods.The stability experiment showed the 3 in 1 cassette can maintain its effect for a long period at the room temperature.Conclusion The detection kit developed by this study is convenient and rapid for detecting ketamine,methamphetamine and morphine in the samples simultaneously.
Keywords:Forensic toxicological analysis  Ketamine  Methamphetamine  Morphine  Colloidal gold  Monoclonal antibody
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号