| DNase-I纯化结合碱性裂解法提取混合斑精子DNA |
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| 引用本文： | 袁自闯,金洪年,赖跃,王慧君,陈红英.DNase-I纯化结合碱性裂解法提取混合斑精子DNA[J].中国法医学杂志,2010(1). |
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| 作者姓名： | 袁自闯 金洪年 赖跃 王慧君 陈红英 |
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| 作者单位： | 南方医科大学法医学系 广东省公安厅刑事技术中心 深圳市公安局刑科所 |
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| 摘 要： | 目的研究脱氧核糖核酸酶I(DNase-I)纯化结合碱性裂解法提取混合斑精子DNA的方法在法医学中的应用。方法收集79份性犯罪案件混合斑检材,分别用DNase-I纯化结合碱性裂解法和差异裂解法提取精子DNA,采用STR荧光标记复合扩增体系进行16个STR基因座分型,并比较检验结果。结果应用DNase-I纯化结合碱性裂解法提取精子DNA,64例检材分型成功;应用差异裂解法提取精子DNA,57例检材分型成功;两种方法比较结果存在显著性差异(P=0.039),DNase-1纯化结合碱性裂解法提取精子DNA的STR分型成功率更高,成本低廉。结论DNase-I纯化结合碱性裂解法提取混合斑精子DNA可提高检验成功率,操作简便,快速,易于自动化,适于法医学个体识别鉴定。
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| 关 键 词： | 法医物证学 脱氧核糖核酸酶I 碱性裂解法 混合斑 精子DNA |
The extraction of sperm DNA from mixed stain using DNase-I purification combined with alkaline lysis method |
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| YUAN Zichuang,;,; JING Hongnian,; LAI Yue,; WANG Huijun,; CHEN Hongying.The extraction of sperm DNA from mixed stain using DNase-I purification combined with alkaline lysis method[J].Chinese Journal of Forensic Medicine,2010(1). |
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| Authors: | YUAN Zichuang ; ; JING Hongnian ; LAI Yue ; WANG Huijun ; CHEN Hongying |
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| Institution: | 1.Department of Forensic Medicine; Southern Medicial University; Guangzhou 510515; Ghina; 2.Guangdong Institution of Criminal Science and Technology; Guangzhou 510050; 3.Shenzhen Institution of Criminal Science and Technology; Guangdong 510840; Ghina); |
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| Abstract: | Objective To establish a method of sperm DNA extraction in mixed stain by using DNase-I purification combined with alkaline lysis method in forensic science.Methods 79 mixed stain samples of criminal cases were collected.Sperm DNA was extracted using the purification of DNase-I binding alkaline lysis method.16 STR loci were genotyped with fluorescent multiplex amplification system.The typing results were compared with that of extracted using two-step differential extraction procedure.Results Of all 79 mixed... |
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| Keywords: | forensic biological evidence DNase-I alkaline lysis method mixed stains sperm DNA |
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