| 用PCR扩增X-Y-特异片段性别鉴定的研究 |
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| 引用本文： | 周静,张纯斌,倪锦堂.用PCR扩增X-Y-特异片段性别鉴定的研究[J].中国法医学杂志,1993(4). |
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| 作者姓名： | 周静 张纯斌 倪锦堂 |
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| 作者单位： | 公安部第二研究所 (北京100038) |
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| 摘 要： | 采用5%Chelex-100处理方法提取 DNA,用 PCR 扩增 Amelogenin 基因片段检测血痕和毛根的性别。扩增一个样品只需1/10体积3mm~2血痕 Chelex-100处理液或1/10体积一根毛发 Chelex-100处理液(含模板 DNA)。100例血液样品结果显示,在男性可同时显现977bpX 特异片段和788bpY 特异片段,而在女性只观察到977bp X 特异片段,该方法用于性别鉴定可靠、灵敏、方便。
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| 关 键 词： | PCR扩增 X-特异片段 Y-特异片段 性别检验 |
A STUDY OF DETERMINATION WITH PCR AMPLIFICATION OF X AND Y CHROMOSOME-SPECIFIC LOCUS |
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| Zhou Jing Zhang Chunbin Ni Jintang The Second Institute of Public Security Ministry,Beijing.A STUDY OF DETERMINATION WITH PCR AMPLIFICATION OF X AND Y CHROMOSOME-SPECIFIC LOCUS[J].Chinese Journal of Forensic Medicine,1993(4). |
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| Authors: | Zhou Jing Zhang Chunbin Ni Jintang The Second Institute of Public Security Ministry Beijing |
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| Institution: | Zhou Jing Zhang Chunbin Ni Jintang The Second Institute of Public Security Ministry,Beijing,100038 |
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| Abstract: | The sex determination of blood stain and plucked hair have been studied with Polymerase ChainReaction(PCR).A pair of PCR primer has been used to amplify the amelogenin gene.We have used5% chelex-100(BIO-Rad Co.USA)to treat the samples for DNA extraction.The sensitivity was 1/10volume of 3 square milimeter blood stain extract or 1/10 volume of a plucked hair chelex extract.Thismethod is sensitive and convenient. |
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| Keywords: | PCR amplification X-specific band Y-specific band Sex determination |
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