| MiniFiler试剂盒进行微量细胞STR分型可行性探讨 |
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| 引用本文： | 苏芹,严红,李彩霞,于子辉,胡兰.MiniFiler试剂盒进行微量细胞STR分型可行性探讨[J].中国法医学杂志,2008,23(5). |
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| 作者姓名： | 苏芹 严红 李彩霞 于子辉 胡兰 |
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| 作者单位： | 1. 中国人民公安大学,北京,100038
2. 公安部物证鉴定中心,北京,100038 |
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| 摘 要： | 目的探讨采用MiniFiler试剂盒进行微量细胞STR分型的可行性。方法利用显微操作法捕获微量口腔上皮细胞,采用MiniFiler试剂盒进行STR复合扩增,ABI 3130遗传分析仪检测STR分型。结果10个口腔上皮细胞能够获得稳定的STR分型;1、3、5个口腔上皮细胞能够获得完整的STR分型,但存在随机性。结论微量细胞进行STR分型具有不稳定性,目前还难以在实际检案中应用,但可以通过增加模板量来提高分型的成功率。
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| 关 键 词： | 法医物证学 显微操作法 MiniFiler试剂盒 口腔上皮细胞 STR |
The exploration of STR genotyping of minute cells by MiniFiler kit |
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| SU Qin,YAN Hong,LI Cai-xia,et al..The exploration of STR genotyping of minute cells by MiniFiler kit[J].Chinese Journal of Forensic Medicine,2008,23(5). |
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| Authors: | SU Qin YAN Hong LI Cai-xia |
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| Institution: | SU Qin,YAN Hong,LI Cai-xia,et al.Chinese Peoples Public Security University,Beijing 100038,China |
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| Abstract: | Objective Explore the feasibility of minute cells STR typing.Methods Minute cells which were obtained by micromanipulation method were amplified with MiniFiler kit,and detected by ABI 3130 genetic analyzer.Results Ten cells can be successfully genotyped.Complete genotyping results can be obtained for one,three or five cells but with stochastic effect.Conclusion Instability is observed in minute cells genotyping,so it can not be used in actual case work.Maybe increase the quantity of DNA template can improve the success rate. |
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| Keywords: | STR |
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